Chemical Labeling of Protein 4'-Phosphopantetheinylation

Chembiochem. 2021 Apr 16;22(8):1357-1367. doi: 10.1002/cbic.202000747. Epub 2021 Jan 26.

Abstract

Nature uses a diverse array of protein post-translational modifications (PTMs) to regulate protein structure, activity, localization, and function. Among them, protein 4'-phosphopantetheinylation derived from coenzyme A (CoA) is an essential PTM for the biosynthesis of fatty acids, polyketides, and nonribosomal peptides in prokaryotes and eukaryotes. To explore its functions, various chemical probes mimicking the natural structure of 4'-phosphopantetheinylation have been developed. In this minireview, we summarize these chemical probes and describe their applications in direct and metabolic labeling of proteins in bacterial and mammalian cells.

Keywords: 4′-phosphopantetheinylation; chemical probes; in situ labeling; in vitro labeling; metabolic labeling.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Review

MeSH terms

  • Coenzyme A / chemistry*
  • Coenzyme A / metabolism
  • Models, Molecular
  • Molecular Structure
  • Pantetheine / analogs & derivatives*
  • Pantetheine / chemistry
  • Pantetheine / metabolism
  • Protein Processing, Post-Translational

Substances

  • Pantetheine
  • 4'-phosphopantetheine
  • Coenzyme A