Molecular investigation on the presence of canine parvovirus in Egypt

Elzahara Elbaz; Mohamed El-Tholoth; Eman A Abo Elfadl; Samah M Mosad

doi:10.1016/j.cimid.2020.101576

Molecular investigation on the presence of canine parvovirus in Egypt

Comp Immunol Microbiol Infect Dis. 2021 Feb:74:101576. doi: 10.1016/j.cimid.2020.101576. Epub 2020 Nov 30.

Authors

Elzahara Elbaz¹, Mohamed El-Tholoth², Eman A Abo Elfadl³, Samah M Mosad⁴

Affiliations

¹ Department of Internal Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Mansoura University, Mansoura, 35516, Egypt.
² Department of Virology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, 35516, Egypt; Health Sciences Division, Veterinary Sciences Program, Al Ain Men's Campus, Higher Colleges of Technology, Al Ain 17155, United Arab Emirates. Electronic address: tholothvirol@mans.edu.eg.
³ Department of Animal Husbandry and Development of Animal Wealth, Faculty of Veterinary Medicine, Mansoura University, Mansoura, 35516, Egypt.
⁴ Department of Virology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, 35516, Egypt.

PMID: 33285387
DOI: 10.1016/j.cimid.2020.101576

Abstract

Canine parvovirus type 2 (CPV-2) causes a highly contagious gastroenteritis disease of dogs and wild canids. To investigate the CPV-2 prevalence in Dakahlia Governorate, Egypt, a total of 50 fecal swabs were collected from suspected diseased dogs during 2016-2017. Out of 50 collected samples, 35 samples (70 %) presented positive results for CPV-2 using immuno-chromatography (IC) as a rapid test. CPV-2DNA was detected in 42 samples (84 %) by using polymerase chain reaction (PCR). The frequencies of CPV-2 were significantly higher in German shepherd breed (46 %; 23/50) and in age groups less than 6 months (76%; 38/50). We evaluated the breed, age, sex, rapid test results and clinical signs as predictors for classification of animal status into infected and not infected. The best predictors for classification process were rapid test result and clinical signs. Both CPV-2b and CPV-2c subtypes were detected by CPV2-VP2 gene sequences analysis. Deduced amino acid sequences alignment showed substitutions at 3 sites (Arg453Pro, Ala574Glu and Gln457Leu). Further investigations are needed to reveal the genetic and antigenic relation between field and vaccinal strains of CPV-2 in Egypt.

Keywords: Canine parvovirus; Dogs; Egypt; PCR; Sequence analysis.

MeSH terms

Animals
Dog Diseases* / diagnosis
Dog Diseases* / epidemiology
Dogs
Egypt / epidemiology
Parvoviridae Infections* / epidemiology
Parvoviridae Infections* / veterinary
Parvovirus, Canine* / genetics
Phylogeny