Interpretation of the HLA epitopes-Continuous dilution method for detection of high-titer IgG HLA antibody

Abstract

Background: The presence or absence of human leukocyte antigen (HLA) antibodies, especially the strength of donor-specific HLA antibodies (DSAs), has important roles in clinical evaluation and diagnostic decision-making for solid-organ transplantation. Dilution patterns help to give a new sight of HLA epitopes. "Epitope matching" is likely to lower the risk of developing DSA and increase the likelihood of matching a compatible donor.

Methods: We collected data evaluating HLA antibodies with a titration study using mean fluorescence intensity.

Results: Diluting the serum of recipients can reduce potential inhibitory effects, accurately evaluate the intensity of donor-specific HLA antibodies, and guide surgeons to take or not take intervention measures. Dilution patterns also help to give a new sight of HLA epitopes.

Conclusion: We believe that from the viewpoint of HLA antibodies, the dilution model can provide new tools and insights for the study of HLA epitopes.

Keywords: donor-specific HLA antibodies; epitope; titration study.