Rheological characterization of Porphyridium sordidum and Porphyridium purpureum exopolysaccharides

Edilberto Vicente Medina-Cabrera; Moritz Gansbiller; Broder Rühmann; Jochen Schmid; Volker Sieber

doi:10.1016/j.carbpol.2020.117237

Rheological characterization of Porphyridium sordidum and Porphyridium purpureum exopolysaccharides

Carbohydr Polym. 2021 Feb 1:253:117237. doi: 10.1016/j.carbpol.2020.117237. Epub 2020 Oct 20.

Authors

Edilberto Vicente Medina-Cabrera¹, Moritz Gansbiller², Broder Rühmann³, Jochen Schmid⁴, Volker Sieber⁵

Affiliations

¹ Technical University of Munich, Campus for Biotechnology and Sustainability, 94315, Straubing, Germany. Electronic address: edilberto.medina-cabrera@tum.de.
² Technical University of Munich, Campus for Biotechnology and Sustainability, 94315, Straubing, Germany. Electronic address: m.gansbiller@tum.de.
³ Technical University of Munich, Campus for Biotechnology and Sustainability, 94315, Straubing, Germany. Electronic address: broder.ruehmann@tum.de.
⁴ Technical University of Munich, Campus for Biotechnology and Sustainability, 94315, Straubing, Germany; Norwegian University of Science and Technology, Department of Biotechnology and Food Science, Sem Sælands vei 6-8, 7491, Trondheim, Norway. Electronic address: jochen.schmid@ntnu.no.
⁵ Technical University of Munich, Campus for Biotechnology and Sustainability, 94315, Straubing, Germany; Catalysis Research Center, Technical University of Munich, 85748, Garching, Germany; Fraunhofer IGB, Branch BioCat, 94315, Straubing, Germany; The University of Queensland, School of Chemistry and Molecular Biosciences, 68 Cooper Road, St. Lucia, 4072, Australia. Electronic address: sieber@tum.de.

PMID: 33278993
DOI: 10.1016/j.carbpol.2020.117237

Abstract

Porphyridium exopolysaccharides (EPSs), which contain sulfate and methyl groups, have a similar potential for use in multiple industrial applications as macroalgae counterparts but lack detailed characterization. For this reason, we produced 0.21 g L^-1 of P. sordidum EPS and 0.17 g L^-1P. purpureum EPS, followed by a thorough rheological characterization in respect to their differences in monomer composition, sulfate concentrations and methyl patterns. Furthermore, the effect of NaCl and CaCl₂ was evaluated, and the effect of high salinity media on the rheological properties of the biopolymers was analyzed. Both Porphyridium EPSs show a remarkable stability at high temperature and under the effect of mono- and divalent cations, and high salinity cultivation medium, which was evidenced by the rheological properties of the EPS. This feature is not displayed by many carbohydrate polymers, making it possible to enrich current applications in which EPS are used.

Keywords: P. purpureum EPS; P. sordidum EPS; Rheological properties; Salts; Stability; Sulfate.

MeSH terms

Biomass
Biopolymers / chemistry
Calcium Chloride / chemistry
Cations / chemistry
Culture Media
Hot Temperature
Plant Extracts / chemistry*
Polysaccharides / chemistry*
Porphyridium / chemistry*
Porphyridium / classification
Rheology / methods*
Salinity
Salts / chemistry
Seaweed / chemistry
Sodium Chloride / chemistry

Substances

Biopolymers
Cations
Culture Media
Plant Extracts
Polysaccharides
Salts
Sodium Chloride
Calcium Chloride