Engineering approaches for effective therapeutic applications based on extracellular vesicles

Seunglee Kwon; Sol Shin; Minjae Do; Byeong Hoon Oh; Yeari Song; Van Dat Bui; Eun Sook Lee; Dong-Gyu Jo; Yong Woo Cho; Deok-Ho Kim; Jae Hyung Park

doi:10.1016/j.jconrel.2020.11.062

Engineering approaches for effective therapeutic applications based on extracellular vesicles

J Control Release. 2021 Feb 10:330:15-30. doi: 10.1016/j.jconrel.2020.11.062. Epub 2020 Dec 2.

Authors

Seunglee Kwon¹, Sol Shin², Minjae Do³, Byeong Hoon Oh¹, Yeari Song¹, Van Dat Bui¹, Eun Sook Lee², Dong-Gyu Jo⁴, Yong Woo Cho⁵, Deok-Ho Kim⁶, Jae Hyung Park⁷

Affiliations

¹ School of Chemical Engineering, College of Engineering, Sungkyunkwan University, Suwon 16419, Republic of Korea.
² Department of Health Sciences and Technology, SAIHST, Sungkyunkwan University, Seoul 06351, Republic of Korea.
³ Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21205, USA.
⁴ Department of Health Sciences and Technology, SAIHST, Sungkyunkwan University, Seoul 06351, Republic of Korea; Biomedical Institute for Convergence at SKKU, Sungkyunkwan University, Suwon 16419, Republic of Korea; School of Pharmacy, Sungkyunkwan University, Suwon 16419, Republic of Korea; ExoStemTech Inc., Ansan 15588, Republic of Korea.
⁵ ExoStemTech Inc., Ansan 15588, Republic of Korea; Department of Chemical Engineering, Hanyang University, Ansan 15588, Republic of Korea.
⁶ Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21205, USA; Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA. Electronic address: dhkim@jhu.edu.
⁷ School of Chemical Engineering, College of Engineering, Sungkyunkwan University, Suwon 16419, Republic of Korea; Department of Health Sciences and Technology, SAIHST, Sungkyunkwan University, Seoul 06351, Republic of Korea; Biomedical Institute for Convergence at SKKU, Sungkyunkwan University, Suwon 16419, Republic of Korea; ExoStemTech Inc., Ansan 15588, Republic of Korea. Electronic address: jhpark1@skku.edu.

PMID: 33278480
DOI: 10.1016/j.jconrel.2020.11.062

Abstract

The biological significance of extracellular vesicles (EVs) as intercellular communication mediators has been increasingly revealed in a wide range of normal physiological processes and disease pathogenesis. In particular, regenerative and immunomodulatory EVs hold potential as innate biotherapeutics, whereas pathological EVs are considered therapeutic targets for inhibiting their bioactivity. Given their ability to transport functional cargos originating from the source cells to target cells, EVs can also be used as a therapeutic means to deliver drug molecules. This review aims to provide an updated overview of the key engineering approaches for better exploiting EVs in disease intervention. The emphasis is lying on the preconditioning methods for therapeutic EVs, drug loading and targeting technologies for carrier EVs, and activity control strategies for pathological EVs.

Keywords: Biogenesis inhibition; Drug loading; Extracellular vesicles; Preconditioning; Surface modification; Therapeutic applications.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Cell Communication
Drug Delivery Systems
Extracellular Vesicles*