Harnessing synthetic biology-based strategies for engineered biosynthesis of nucleoside natural products in actinobacteria

Rong Gong; Le Yu; Yini Qin; Neil P J Price; Xinyi He; Zixin Deng; Wenqing Chen

doi:10.1016/j.biotechadv.2020.107673

Harnessing synthetic biology-based strategies for engineered biosynthesis of nucleoside natural products in actinobacteria

Biotechnol Adv. 2021 Jan-Feb:46:107673. doi: 10.1016/j.biotechadv.2020.107673. Epub 2020 Dec 1.

Authors

Rong Gong¹, Le Yu¹, Yini Qin¹, Neil P J Price², Xinyi He³, Zixin Deng⁴, Wenqing Chen⁵

Affiliations

¹ Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, and School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, China.
² US Department of Agriculture, Agricultural Research Service, National Center for Agricultural Utilization Research, Peoria, IL, USA.
³ State Key Laboratory of Microbial Metabolism, and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
⁴ Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, and School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, China; State Key Laboratory of Microbial Metabolism, and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China. Electronic address: zxdeng@sjtu.edu.cn.
⁵ Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, and School of Pharmaceutical Sciences, Wuhan University, Wuhan 430071, China. Electronic address: wqchen@whu.edu.cn.

PMID: 33276073
DOI: 10.1016/j.biotechadv.2020.107673

Abstract

Antibiotic resistance poses an increasing threat to global health, and it is urgent to reverse the present trend by accelerating development of new natural product derived drugs. Nucleoside antibiotics, a valuable family of promising natural products with remarkable structural features and diverse biological activities, have played significant roles in healthcare and for plant protection. Understanding the biosynthesis of these intricate molecules has provided a foundation for bioengineering the microbial cell factory towards yield enhancement and structural diversification. In this review, we summarize the recent progresses in employing synthetic biology-based strategies to improve the production of target nucleoside antibiotics. Moreover, we delineate the advances on rationally accessing the chemical diversities of natural nucleoside antibiotics.

Keywords: Chemical diversities; Microbial cell factory; Natural product; Nucleoside antibiotics; Synthetic biology-based strategies; Yield enhancement.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Actinobacteria* / genetics
Anti-Bacterial Agents
Biological Products*
Nucleosides
Synthetic Biology

Substances

Anti-Bacterial Agents
Biological Products
Nucleosides