Hydroxyapatite-decorated ZrO2 for α-amylase immobilization: Toward the enhancement of enzyme stability and reusability

Yaaser Q Almulaiky; N M Khalil; Reda M El-Shishtawy; Tariq Altalhi; Yousif Algamal; Musab Aldhahri; Sami A Al-Harbi; Esam S Allehyani; Muhammad Bilal; Mustafa M Mohammed

doi:10.1016/j.ijbiomac.2020.11.150

Hydroxyapatite-decorated ZrO₂ for α-amylase immobilization: Toward the enhancement of enzyme stability and reusability

Int J Biol Macromol. 2021 Jan 15:167:299-308. doi: 10.1016/j.ijbiomac.2020.11.150. Epub 2020 Dec 1.

Authors

Affiliations

¹ University of Jeddah, College of Sciences and Arts at Khulais, Department of Chemistry, Jeddah, Saudi Arabia; Chemistry Department, Faculty of Applied Science, Taiz University, Taiz, Yemen. Electronic address: yaseralmoliki@hotmail.com.
² University of Jeddah, College of Sciences and Arts at Khulais, Department of Chemistry, Jeddah, Saudi Arabia; Refractories, Ceramics and Building Materials Department, National Research Centre, Dokki, Cairo 12622, Egypt.
³ Chemistry Department, Faculty of Science, King Abdulaziz University, Jeddah, P. O. Box 80200, 21589, Saudi Arabia; Dyeing, Printing, and Textile Auxiliaries Department, Textile Research Division, National Research Centre, Dokki, Cairo 12622, Egypt. Electronic address: relshishtawy@kau.edu.sa.
⁴ Department of Chemistry, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia.
⁵ University of Jeddah, College of Sciences and Arts at Khulais, Department of Chemistry, Jeddah, Saudi Arabia; Chemistry Department, Faculty of Science & Technology, Omdurman Islamic University. Sudan.
⁶ Department of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah, P. O. Box 80200, 21589, Saudi Arabia; Center of Nanotechnology, King Abdulaziz University, Jeddah 21589, Saudi Arabia.
⁷ Department of Chemistry, University College in Al-Jamoum, Umm Al-Qura University, Makkah, Saudi Arabia.
⁸ School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huaian 223003, China.
⁹ Department of Mathematics, College of Sciences & Arts - Khulais, University of Jeddah, Jeddah, Saudi Arabia; Department of statistics, Faculty of science, Sudan University of Science and Technology, Khartoum, Sudan.

PMID: 33275970
DOI: 10.1016/j.ijbiomac.2020.11.150

Abstract

Herein, the immobilization of α-amylase onto hydroxyapatite (HA) and hydroxyapatite-decorated ZrO₂ (10%wt) (HA-ZrO₂) nanocomposite were investigated. The immobilization yield was 69.7% and 84% respectively. The structural differences were characterized using X-Ray diffraction, attenuated total reflectance-Fourier transform infrared spectra, Raman, and scanning electron microscope. After 10 repeated cycles, the residual activity of immobilized α-amylase onto HA and HA-ZrO₂ nanocomposite was 46% and 70%, respectively. The storage stability was recorded to be 27%, 50% and 69% from its initial activity in the case of free and immobilized enzyme onto HA and HA-ZrO₂ nanocomposite, respectively after 8 weeks. The pH-activity profile and temperature revealed pH 6.0 and temperature 50 °C as the optimal values of free α-amylase, while the optimum values for α-amylase on HA and HA-ZrO₂ was shifted to pH 6.5 and 60 °C after immobilization. The immobilized α-amylase onto HA-ZrO₂ showed comparatively higher catalytic activity than the free α-amylase. The Km value after the immobilization process onto HA was 2.1 folds highly than that of the free enzyme. In conclusion, it can be inferred that HA-ZrO₂ is more sustainable and beneficial support for enzyme immobilization and it represents promising supports for different uses of α-amylase in the biomedical applications.

Keywords: Hydroxyapatite-ZrO(2); Reusability; α-Amylase.

MeSH terms

Biocatalysis
Chemical Phenomena
Durapatite / chemistry*
Enzyme Stability
Enzymes, Immobilized*
Hydrogen-Ion Concentration
Nanoparticles / chemistry
Nanoparticles / ultrastructure
Spectroscopy, Fourier Transform Infrared
Spectrum Analysis, Raman
Temperature
X-Ray Diffraction
Zirconium / chemistry*
alpha-Amylases / chemistry*

Substances

Enzymes, Immobilized
Durapatite
Zirconium
alpha-Amylases
zirconium oxide