A plasmonic biomimetic enzyme-linked immunosorbent assay (PBELISA) method was developed for ultrasensitive and on-site visual detection of bisphenol A (BPA). The PBELISA was an enzyme-linked immunoassay using molecularly imprinted polymer (MIP) film as biomimetic antibody combined with catalase (CAT)-mediated growth of plasmonic gold nanoparticles (AuNPs). With the BPA concentration increased, a distinguished color change was observed from colorless to blue and then red. Therefore, the proposed method could be employed with naked-eye observation to detect BPA with visual limit of detection (LOD) of 40 pg/mL. For quantitative analysis, this method also exhibited a good dynamic linear response to the logarithmic BPA concentrations ranged from 10 pg/mL to 1.024 × 104 pg/mL with a correlation coefficient of R2 = 0.9922 and LOD of 6.20 pg/mL. The recovery rates in tap water, milk and orange juice ranged from 91.83% to 107.39%. In brief, the developed PBELISA method is sensitive, cost-effective and easy-to-use for BPA detection.
Keywords: Bisphenol A; Enzyme-linked immunosorbent assay; Gold nanoparticle; Molecularly imprinted polymer; Surface plasmon resonance.
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