Illumina sequencing revealed roles of microRNAs in different aluminum tolerance of two citrus species

Yang-Fei Zhou; Yan-Yu Wang; Wei-Wei Chen; Li-Song Chen; Lin-Tong Yang

doi:10.1007/s12298-020-00895-y

Illumina sequencing revealed roles of microRNAs in different aluminum tolerance of two citrus species

Physiol Mol Biol Plants. 2020 Nov;26(11):2173-2187. doi: 10.1007/s12298-020-00895-y. Epub 2020 Oct 27.

Authors

Yang-Fei Zhou¹, Yan-Yu Wang¹, Wei-Wei Chen¹, Li-Song Chen^{1

2}, Lin-Tong Yang^{1

2}

Affiliations

¹ College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou, 350002 China.
² Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation, Fujian Agriculture and Forestry University, Fuzhou, 350002 China.

Abstract

Self-germinated seedlings of Citrus sinensis and C. grandis were supplied with nutrient solution with 0 mM AlCl₃·6H₂O (control, -Al) or 1 mM AlCl₃·6H₂O (+Al) for 18 weeks. The DW (Dry weights) of leaf, stem, shoot and the whole plant of C. grandis were decreased and the ratio of root DW to shoot DW in C. grandis were increased by Al, whereas these parameters of C. sinensis were not changed by Al. Al treatment dramatically decreased the sulfur (S) content in C. grandis roots and the phosphorus (P) content in both C. sinensis and C. grandis roots. More Al was transported to shoots and leaves in C. grandis than in C. sinensis under Al treatment. Al treatment has more adverse effects on C. grandis than on C. sinensis, as revealed by the higher production of superoxide anion (O₂ ^·-), H₂O₂ and thiobarbituric acid reactive substace (TBARS) content in C. grandis roots. Via the Illumina sequencing technique, we successfully identified and quantified 12 and 16 differentially expressed miRNAs responding to Al stress in C. sinensis and C. grandis roots, respectively. The possible mechanism underlying different Al tolerance of C. sinensis and C. grandis were summarized as having following aspects: (a) enhancement of adventitious and lateral root development (miR160); (b) up-regulation of stress and signaling transduction related genes, such as SGT1, PLC and AAO (miR477, miR397 and miR398); (c) enhancement of citrate secretion (miR3627); (d) more flexible control of alternative glycolysis pathway and TCA cycle (miR3627 and miR482); (e) up-regulation of S-metabolism (miR172); (f) more flexible control of miRNA metabolism. For the first time, we showed that root development (miR160) and cell wall components (cas-miR5139, csi-miR12105) may play crucial roles in Al tolerance in citrus plants. In conclusion, our study provided a comprehensive profile of differentially expressed miRNAs in response to Al stress between two citrus plants differing in Al tolerance which further enriched our understanding of the molecular mechanism underlying Al tolerance in plants.

Keywords: Aluminum toxicity; Antioxidant system; Biological regulation; Citrus; miRNA.