Confirmation of five nitrofuran metabolites including nifursol metabolite in meat and aquaculture products by liquid chromatography-tandem mass spectrometry: Validation according to European Union Decision 2002/657/EC

Pierre Guichard; Michel Laurentie; Dominique Hurtaud-Pessel; Eric Verdon

doi:10.1016/j.foodchem.2020.128389

Confirmation of five nitrofuran metabolites including nifursol metabolite in meat and aquaculture products by liquid chromatography-tandem mass spectrometry: Validation according to European Union Decision 2002/657/EC

Food Chem. 2021 Apr 16:342:128389. doi: 10.1016/j.foodchem.2020.128389. Epub 2020 Oct 15.

Authors

Pierre Guichard¹, Michel Laurentie², Dominique Hurtaud-Pessel³, Eric Verdon⁴

Affiliations

¹ ANSES Fougeres Laboratory, French National Agency for Food, Environment and Occupational Health & Safety, French and European Union Reference Laboratory for Veterinary Medicinal Product Residues and Pharmacologically Active Dye Residues in Food, 10 B rue Claude Bourgelat - Javené, CS 40608, 35306 Fougères Cedex, France. Electronic address: pierre.guichard@anses.fr.
² ANSES Fougeres Laboratory, French National Agency for Food, Environmental and Occupational Health & Safety & Safety, National Methodology Platform for Statistical Support (PAS) on Reference Activities, 10 B rue Claude Bourgelat - Javené, CS 40608, 35306 Fougères Cedex, France. Electronic address: michel.laurentie@anses.fr.
³ ANSES Fougeres Laboratory, French National Agency for Food, Environment and Occupational Health & Safety, French and European Union Reference Laboratory for Veterinary Medicinal Product Residues and Pharmacologically Active Dye Residues in Food, 10 B rue Claude Bourgelat - Javené, CS 40608, 35306 Fougères Cedex, France. Electronic address: dominique.pessel@anses.fr.
⁴ ANSES Fougeres Laboratory, French National Agency for Food, Environment and Occupational Health & Safety, French and European Union Reference Laboratory for Veterinary Medicinal Product Residues and Pharmacologically Active Dye Residues in Food, 10 B rue Claude Bourgelat - Javené, CS 40608, 35306 Fougères Cedex, France. Electronic address: eric.verdon@anses.fr.

PMID: 33268173
DOI: 10.1016/j.foodchem.2020.128389

Abstract

LC-MS/MS method for confirmation of nitrofuran metabolites in meat and aquaculture products, including the nifursol metabolite (DNSH), was developed. The nitrofuran metabolites investigated were as follows: 3-amino-2-oxazolidinone (AOZ), 3-amino-5-morpholinomethyl-2-oxazolidinone (AMOZ), 1-aminohydantoine (AHD), semicarbazide (SEM) and 3,5-dinitrosalicylic acid hydrazide (DNSH). The sample preparation includes a washing step, allowing to analyze only the fraction of protein-bound residues. The final optimized recovery solvent for injection was found to be a mixture of ammonium acetate 2 mM/acetonitrile (60/40 ; v/v). Matrix effects and stability in biological matrix and standard solution for DNSH have been also carried out. Method performances were assessed using criteria of the Decision (EC) No 2002/657 and considering the proposed-for-adoption reference point for action (RPA) of 0.50 µg.kg^-1 under Reg 1871/2019. Trueness ranged 86.5%-103.7% and precision ranged 2.0%-6.5% on intra-laboratory reproducibility conditions. Decision limit (CCα) ranged 0.028-0.182 μg.kg^-1 and capability of detection limit (CCβ) ranged 0.032-0.233 µg.kg^-1.

Keywords: Liquid chromatography/mass spectrometry (LC-MS/MS); Nifursol metabolite; Nitrofuran metabolites; Prohibited antimicrobials; Veterinary residues.

MeSH terms

Animals
Chromatography, High Pressure Liquid / standards
Limit of Detection
Meat / analysis*
Muscles / chemistry
Muscles / metabolism
Nitrofurans / analysis*
Nitrofurans / metabolism
Nitrofurans / standards
Reference Standards
Reproducibility of Results
Tandem Mass Spectrometry / methods*
Tandem Mass Spectrometry / standards

Substances

Nitrofurans