Transcriptome profiling of Pseudomonas aeruginosa YH reveals mechanisms of 2, 2', 4, 4'-tetrabrominated diphenyl ether tolerance and biotransformation

Shaoyu Tang; Hua Yin; Xiaolong Yu; Shuona Chen; Guining Lu; Zhi Dang

doi:10.1016/j.jhazmat.2020.124038

Transcriptome profiling of Pseudomonas aeruginosa YH reveals mechanisms of 2, 2', 4, 4'-tetrabrominated diphenyl ether tolerance and biotransformation

J Hazard Mater. 2021 Feb 5:403:124038. doi: 10.1016/j.jhazmat.2020.124038. Epub 2020 Sep 18.

Authors

Shaoyu Tang¹, Hua Yin², Xiaolong Yu³, Shuona Chen⁴, Guining Lu¹, Zhi Dang¹

Affiliations

¹ Key Laboratory of Ministry of Education on Pollution Control and Ecosystem Restoration in Industry Clusters, School of Environment and Energy, South China University of Technology, Guangzhou 510006, Guangdong, China.
² Key Laboratory of Ministry of Education on Pollution Control and Ecosystem Restoration in Industry Clusters, School of Environment and Energy, South China University of Technology, Guangzhou 510006, Guangdong, China. Electronic address: huayin@scut.edu.cn.
³ Guangdong Provincial Key Laboratory of Petrochemical Pollution Processes and Control, School of Environmental Science and Engineering, Guangdong University of Petrochemical Technology, Maoming, Guangdong 525000, China.
⁴ College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China.

PMID: 33265052
DOI: 10.1016/j.jhazmat.2020.124038

Abstract

Aerobic degradation of 2, 2', 4, 4'-tetrabrominated diphenyl ether (BDE-47) by Pseudomonas aeruginosa YH (P. aeruginosa YH) were investigated in this study. BDE-47 degradation was mainly through the biological action of intracellular enzymes, and the metabolites included debrominated metabolites (BDE-28 and BDE-7), hydroxylated metabolites (6-OH-BDE-47, 5-OH-BDE-47, 2'-OH-BDE-28 and 4'-OH-BDE-17), and brominated phenols (2,4-DBP and 4-BP). P. aeruginosa YH also exhibited exceptional ability to degrade intermediates, and the degradation rates of 50 μg/L BDE-28, BDE-7, and 2,4-DBP were 68.4%, 82.3% and 92.7% on the 5th day, separately. Transcriptome sequencing revealed that 991 genes were up-regulated, and 923 genes were down-regulated in P. aeruginosa YH after exposure to 0.5 mg/L BDE-47 (FDR ≤ 0.001, |log₂Ratio| ≥ 1). The differentially expressed genes were related to transport, metabolism and stress response. Harf inhibitory concentration (IC₅₀) of BDE-47 decreased from 167.5 mg/L to 68.4 mg/L when multidrug efflux pump was inactivated by 20 mg/L andrographolide, indicating that it helped the bacterial tolerance against BDE-47. Moreover, efflux pump inhibition would accelerate the adsorption of BDE-47. The adsorption rate obtained equilibrium at approximately 70% in 2 days, while 5 days in the control group. Degradation efficiency of 2 mg/L BDE-47 decreased from 26.8% to 13.9% when multidrug efflux was suppressed.

Keywords: Biodegradation; Cellular distribution; Multidrug efflux pump; Polybrominated diphenyl ethers; Transcriptome sequencing.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biotransformation
Gene Expression Profiling
Halogenated Diphenyl Ethers*
Pseudomonas aeruginosa* / genetics

Substances

Halogenated Diphenyl Ethers