Characterization of a novel genomovirus in the phytopathogenic fungus Botrytis cinerea

Fangmin Hao; Mingde Wu; Guoqing Li

doi:10.1016/j.virol.2020.11.007

Characterization of a novel genomovirus in the phytopathogenic fungus Botrytis cinerea

Virology. 2021 Jan 15:553:111-116. doi: 10.1016/j.virol.2020.11.007. Epub 2020 Nov 24.

Authors

Fangmin Hao¹, Mingde Wu², Guoqing Li³

Affiliations

¹ The State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei, 430070, China; The Key Laboratory of Plant Pathology of Hubei Province, Huazhong Agricultural University, Wuhan, Hubei, 430070, China; Institute of Vegetables and the Key Lab of Cucurbitaceous Vegetables Breeding in Ningbo City, Ningbo Academy of Agricultural Sciences, Ningbo, 315040, PR China.
² The State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei, 430070, China; The Key Laboratory of Plant Pathology of Hubei Province, Huazhong Agricultural University, Wuhan, Hubei, 430070, China. Electronic address: mingde@mail.hzau.edu.cn.
³ The State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei, 430070, China; The Key Laboratory of Plant Pathology of Hubei Province, Huazhong Agricultural University, Wuhan, Hubei, 430070, China.

PMID: 33264653
DOI: 10.1016/j.virol.2020.11.007

Abstract

This study characterized a single-stranded circular DNA virus in Botrytis cinerea-namely, Botrytis cinerea genomovirus 1 (BcGV1). The genome of BcGV1 was 1710 nucleotides (nts) long, possessing two ORFs, encoding a putative replication initiation protein (Rep) and a hypothetical protein. The Rep contained seven conserved motifs. The two ORFs were separated by two intergenic regions; the large intergenic region (LIR) contained 259 nts while the small intergenic region (SIR) contained 95 nts. A nonanucleotide, TAACAGTAC, in the LIR was predicted to be associated with the initiation of viral replication. Based on the phylogenetic tree constructed by Reps, BcGV1 belongs to the family Genomoviridae, forming an independent branch, indicating that BcGV1 may belong to a new genus. BcGV1 could be detected in 6.7% of tested B. cinerea strains, suggesting that BcGV1 may be widely distributed in the Chinese B. cinerea population.

Keywords: Botrytis cinerea; Genomoviridae; Mycovirus; ssDNA.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Botrytis / virology*
China
DNA Viruses / classification*
DNA Viruses / genetics
DNA Viruses / isolation & purification*
DNA Viruses / physiology
Fungal Viruses / classification*
Fungal Viruses / genetics
Fungal Viruses / isolation & purification*
Fungal Viruses / physiology
Genome, Viral
Open Reading Frames
Phylogeny
Viral Proteins / genetics
Viral Proteins / metabolism
Virus Replication

Substances

Viral Proteins

Supplementary concepts

Botrytis cinerea