Isolation, structure identification and hepatoprotective activity of a polysaccharide from Sabia parviflora

Bioorg Med Chem Lett. 2021 Jan 15:32:127719. doi: 10.1016/j.bmcl.2020.127719. Epub 2020 Nov 27.

Abstract

The extraction, purification, structure and hepatoprotective activity of a homogenous polysaccharide (SPS60) from Sabia parviflora were investigated. SPS60 was screened after purification with Sephadex G-100 and showed the excellent hepatoprotective activity. Its structural characteristics were investigated by Time of flight mass spectrometry (TOF-MS), PMP Pre-column derivatization-HPLC (PMP-HPLC), nuclear magnetic resonance (NMR) spectroscopy and Atomic Force Microscopy (AFM). The results showed that SPS60 possessed the molecular weight of 16900 Da and the monosaccharide component was glucose, as well as a 1 → 6 glycosidic bond. The results of atomic force microscopy (AFM) show that SPS60 is a blocky sphere in solution. Furthermore, the SPS60 could significantly improve the survival rate of LO2 hepatocytes which were damaged by CCl4. Therefore, SPS60 may be an active substance of S. parviflora as a local functional tea.

Keywords: Extraction and structure; Hepatoprotective activity; Sabia parviflora polysaccharide.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line
  • Cell Survival / drug effects
  • Humans
  • Magnoliopsida / metabolism*
  • Plant Leaves / metabolism
  • Plant Stems / metabolism
  • Polysaccharides / chemistry*
  • Polysaccharides / isolation & purification
  • Polysaccharides / pharmacology
  • Protective Agents / chemistry*
  • Protective Agents / isolation & purification
  • Protective Agents / pharmacology

Substances

  • Polysaccharides
  • Protective Agents