Sleeve Gastrectomy in Mice using Surgical Clips

J Vis Exp. 2020 Nov 14:(165). doi: 10.3791/60719.

Abstract

The number of people who are overweight and obese is continually increasing both in the adult and adolescent populations. This coincides with the increased universal phenomenon of type 2 diabetes (T2D) and other metabolic problems. Bariatric surgery, such as SG, is currently one of the most effective and commonly used long-term treatment for obesity and T2D, but the association between them is not completely explored yet. The mechanisms underlying the outcomes seen after bariatric surgery in humans can be investigated based on preclinical animal studies. The SG reduces body weight, glucose levels and many metabolic parameters, and is easy to perform with a low incidence of complications. The goal of this work is to provide a simple method and an uncomplicated preclinical model of bariatric surgery in animals for researchers.

Publication types

  • Video-Audio Media

MeSH terms

  • Animals
  • Body Weight
  • Cholesterol / blood
  • Diabetes Mellitus, Type 2 / complications
  • Gastrectomy / methods*
  • Glucose Tolerance Test
  • Glycated Hemoglobin / metabolism
  • Insulin Resistance
  • Male
  • Mice, Inbred C57BL
  • Stomach / surgery
  • Surgical Instruments*
  • Treatment Outcome

Substances

  • Glycated Hemoglobin A
  • Cholesterol