An Acute, Rather Than Progressive, Increase in Temperature-Humidity Index Has Severe Effects on Mortality in Laying Hens

Seokmin Kang; Da-Hye Kim; Sang Lee; Taehoon Lee; Kyung-Woo Lee; Hong-Hee Chang; Byunghern Moon; Tugay Ayasan; Yang-Ho Choi

doi:10.3389/fvets.2020.568093

An Acute, Rather Than Progressive, Increase in Temperature-Humidity Index Has Severe Effects on Mortality in Laying Hens

Front Vet Sci. 2020 Nov 5:7:568093. doi: 10.3389/fvets.2020.568093. eCollection 2020.

Authors

Seokmin Kang¹, Da-Hye Kim², Sang Lee², Taehoon Lee³, Kyung-Woo Lee², Hong-Hee Chang^{3

4}, Byunghern Moon⁵, Tugay Ayasan⁶, Yang-Ho Choi^{1

3

4}

Affiliations

¹ Division of Applied Life Sciences (BK21 Plus Program), Gyeongsang National University, Jinju, South Korea.
² Department of Animal Science and Technology, Konkuk University, Seoul, South Korea.
³ Department of Animal Science, Gyeongsang National University, Jinju, South Korea.
⁴ Institute of Agriculture and Life Sciences, Gyeongsang National University, Jinju, South Korea.
⁵ Celltech Co., Ltd., Chungbuk, South Korea.
⁶ Kadirli Academy of Applied Science, Osmaniye Korkut Ata University, Osmaniye, Turkey.

Abstract

This study was conducted to evaluate the effects of temperature-humidity index (THI) on the mortality and the panting rates in hens exposed to varying thermal environments. Hens were challenged with an acute elevation in THI in Experiment 1, where dry-bulb temperature and relative humidity were set at ~27°C and 56% at the beginning of the experiment and changed to 36°C and 45% at its conclusion, respectively. In Experiment 2, different groups of hens were exposed to a progressive increase in THI, with similar ranges to those used in the previous experiment. In Experiment 3, the hens used in Experiment 2 were again challenged by THI conditions, the intensity of which ranged between those used in the previous two experiments. In Experiment 4, panting rates were recorded under varying THI. In the last, plasma biochemical profiles were determined in blood taken from hens subjected to experimental conditions similar to those in Experiment 2. When THI was acutely elevated from 24.2° to 32.1°C within 1 h and then maintained over 4.5 h, no mortality was detected in the first hour, but exceeded 95% after 5 h, and reached 100% at 5.5 h. A gradual increase in THI to 31.2°C over 6 h did not result in mortality during the first 3 h. When THI was set below the conditions in Experiment 1 but above those in Experiment 2, mortality was 29% at 4 h, 75% at 5 h, and 79% at 8 h. However, no mortality was detected in their respective control groups. Panting was not observed under 25.3°C and was largely variable under 30°C. However, all hens exhibited panting exceeding 250 counts/min and 60% mortality at 34°C when heat stress continued for a duration of up to 280 min. In Experiment 5, high ambient THI resulted in significant reductions in plasma albumin, amylase and aspartate aminotransferase, compared with those in control group (P < 0.05). These results suggest that an acute elevation of THI has more severe effects on mortality in hens than gradual changes even when temperature and humidity are similar in both cases.

Keywords: heat stress; laying hens; mortality; panting; temperature-humidity index (THI).