Isolation, characterization and genome sequencing of the novel phage SL25 from the Yellow Sea, China

Zhaoyang Liu; Huifang Li; Min Wang; Yong Jiang; Qingwei Yang; Xinhao Zhou; Zheng Gong; Qian Liu; Hongbing Shao

doi:10.1016/j.margen.2017.09.008

Isolation, characterization and genome sequencing of the novel phage SL25 from the Yellow Sea, China

Mar Genomics. 2018 Feb:37:31-34. doi: 10.1016/j.margen.2017.09.008. Epub 2017 Oct 16.

Authors

Zhaoyang Liu¹, Huifang Li¹, Min Wang², Yong Jiang³, Qingwei Yang¹, Xinhao Zhou¹, Zheng Gong¹, Qian Liu¹, Hongbing Shao¹

Affiliations

¹ College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China.
² College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China; Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao 266003, China. Electronic address: mingwang@ouc.edu.cn.
³ College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China; Institute of Evolution and Marine Biodiversity, Ocean University of China, Qingdao 266003, China. Electronic address: yongjiang@ouc.edu.cn.

PMID: 33250123
DOI: 10.1016/j.margen.2017.09.008

Abstract

Outnumbering all other biological entities on earth, bacteriophages play critical roles in structuring microbial communities. However, only a small number of phages have so far been reported. In this study, a novel Pseudoalteromonas phage, SL25, was isolated from the yellow sea, China. Transmission electron microscope observations showed that phage has an icosahedral head, 100±1nm in diameter, and a tail with a length of 150±1nm, and should be grouped into the Siphoviridae family. To better understand the genetic diversity of this phage, the complete genome was characterized. It consists 29,130-bp-length double-stranded DNA with a GC content of 41.04% and is predicted to have 61 open reading frames (ORFs) with an average length of 504 nucleotides. This study adds a new Siphoviridae phage to the marine bacteriophage dataset that could potentially infect Pseudoalteromonas. It also provides useful data for further molecular research on the interaction mechanism between bacteriophages and their hosts.

Keywords: Complete genome; Pseudoalteromonas phage; Siphoviridae.