The impact of Sample Handling Time on metformin serum concentration

Edyta Sutkowska; Paulina Fortuna; Bernadetta Kałuża; Karolina Sutkowska; Paweł Hodurek; Mariusz G Fleszar

doi:10.1016/j.biopha.2020.110971

The impact of Sample Handling Time on metformin serum concentration

Biomed Pharmacother. 2021 Jan:133:110971. doi: 10.1016/j.biopha.2020.110971. Epub 2020 Nov 25.

Authors

Edyta Sutkowska¹, Paulina Fortuna², Bernadetta Kałuża³, Karolina Sutkowska⁴, Paweł Hodurek⁵, Mariusz G Fleszar⁶

Affiliations

¹ Department and Division of Medical Rehabilitation, Wroclaw Medical University, Poland. Electronic address: edyta.sutkowska@umed.wroc.pl.
² Department of Medical Biochemistry, Wroclaw Medical University, Poland. Electronic address: paulina.fortuna@umed.wroc.pl.
³ Department of Internal Medicine, Endocrinology and Diabetology, Central Clinical Hospital of the Ministry of the Interior, Warsaw, Poland. Electronic address: bernadettta@o2.pl.
⁴ Faculty of Medicine, Wroclaw Medical University, Poland. Electronic address: karolina.sutkowska@student.umed.wroc.pl.
⁵ Department of Medical Biochemistry, Wroclaw Medical University, Poland. Electronic address: pawel.hodurek@umed.wroc.pl.
⁶ Department of Medical Biochemistry, Wroclaw Medical University, Poland; Łukasiewicz Research Network, PORT Polish Center for Technology Development, Poland. Electronic address: mariusz.fleszar@umed.wroc.pl.

PMID: 33248407
DOI: 10.1016/j.biopha.2020.110971

Abstract

This is an informative article which can help research providers to arrange and conduct studies dedicated to the assessment of metformin serum concentrations. If there is a problem with coordination of sample preparation and it is necessary to measure metformin concentration, two hours gap between blood drain and centrifugation has no impact on the results.

Keywords: Centrifugation; Metformin serum concentration; Sample Handling Time.

MeSH terms

Blood Specimen Collection*
Centrifugation
Chromatography, Liquid
Diabetes Mellitus, Type 2 / blood*
Diabetes Mellitus, Type 2 / drug therapy
Drug Monitoring*
Humans
Hypoglycemic Agents / blood*
Hypoglycemic Agents / therapeutic use
Mass Spectrometry
Metformin / blood*
Metformin / therapeutic use
Proof of Concept Study
Reproducibility of Results
Time Factors
Workflow

Substances

Hypoglycemic Agents
Metformin