Development and properties of endodontic resin sealers with natural oils

Cristiane M Reiznautt; Juliana S Ribeiro; Everton Kreps; Wellington L O da Rosa; Hellen de Lacerda; Sonia L Peralta; Marco C Bottino; Rafael G Lund

doi:10.1016/j.jdent.2020.103538

Development and properties of endodontic resin sealers with natural oils

J Dent. 2021 Jan:104:103538. doi: 10.1016/j.jdent.2020.103538. Epub 2020 Nov 26.

Authors

Cristiane M Reiznautt¹, Juliana S Ribeiro¹, Everton Kreps¹, Wellington L O da Rosa¹, Hellen de Lacerda¹, Sonia L Peralta², Marco C Bottino³, Rafael G Lund⁴

Affiliations

¹ Department of Restorative Dentistry, School of Dentistry, UFPel-Federal University of Pelotas, Pelotas, Rio Grande do Sul, Brazil.
² Faculdade Metropolitana da Grande Fortaleza, Programa de Graduação em Odontologia, Fortaleza, Ceará, Brazil.
³ University of Michigan School of Dentistry, Department of Cariology, Restorative Sciences, and Endodontics, 1011N. University (Room 5223), Ann Arbor, MI, 48109, USA. Electronic address: mbottino@umich.edu.
⁴ Department of Restorative Dentistry, School of Dentistry, UFPel-Federal University of Pelotas, Pelotas, Rio Grande do Sul, Brazil. Electronic address: rafael.lund@gmail.com.

PMID: 33248212
DOI: 10.1016/j.jdent.2020.103538

Abstract

Objectives: To synthesize and evaluate the physicochemical, antimicrobial, and cytocompatibility properties of experimental resin-based endodontic sealers containing butia or copaiba natural oils.

Methods: Experimental groups contained butia (Butia capitata) at 0.5 % (B0.5), 1 % (B1), 2 % (B2), and copaiba (Copaifera spp.) at 0.5 % (C0.5), 1 % (C1), and 2 % (C2). The control group contained no added oils (experimental material, EM) and the commercial group was a methacrylate-based resin material (RealSeal™, SybronEndo Corporation, Orange, CA, USA). Degree of conversion, film thickness, setting time, flow, water sorption and solubility, and radiopacity were measured. Meanwhile, antimicrobial (modified direct contact test) and cytotoxicity assays of the experimental groups and controls were performed. One-way ANOVA was used to determine the effect of the independent variable (material) on the degree of conversion, film thickness, radiopacity, flow, setting time, water sorption and solubility, and cytotoxicity. For antimicrobial assays, data were analyzed using two-way ANOVA and Ŝidák's test.

Results: The experimental groups containing natural oils showed higher values of degree of conversion, and lowest water sorption and solubility. EM, B0.5, B1, B2, and RS showed similar film thicknesses. The flow values were statistically similar in all groups. The experimental groups showed adequate cell compatibility. Copaiba oil at 2% increased the antimicrobial effect after 1 and 24 h (p < 0.05). The incorporation of butia or copaiba resulted in a slight modification in some physicochemical properties of the experimental resin sealers.

Conclusion: Novel resin sealers containing natural oils are a promising alternative for endodontics, because of their good physicochemical properties, antimicrobial effects, and cytocompatibility when compared to a commercially available sealer.

Clinical significance: Endodontic sealers containing butia or copaiba had satisfactory cytocompatibility, antimicrobial effects, and adequate physicochemical properties.

Keywords: Antimicrobial; Butia; Copaiba; Enterococcus faecalis; Essential oils; Resin sealer.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Infective Agents* / pharmacology
Materials Testing
Methacrylates
Oils, Volatile*
Root Canal Filling Materials* / pharmacology

Substances

Anti-Infective Agents
Methacrylates
Oils, Volatile
Root Canal Filling Materials