Insertion sequences disrupting mgrB in carbapenem-resistant Klebsiella pneumoniae strains in Brazil

J Glob Antimicrob Resist. 2021 Mar:24:53-57. doi: 10.1016/j.jgar.2020.11.003. Epub 2020 Nov 24.

Abstract

Objectives: This study aimed to characterise insertional mutations disturbing themgrB gene in carbapenem-resistant Klebsiella pneumoniae (CRKp).

Methods: A total of 118 clinical CRKp isolates were surveyed for polymyxin resistance and insertion sequence (IS) elements disruptingmgrB.

Results: Of the 118 isolates, 78 (66.1%) displayed polymyxin resistance, of which 54% (42/78) hadmgrB::IS inserts. Sequencing analyses showed 13 insertion sites in mgrB. mgrB::ISSen4(IS3) was observed for the first time in CRKp.

Conclusions: Ten different IS elements disruptedmgrB, with a predominance (76%) of IS5 sequences.

Keywords: Insertion sequence; Polymyxin resistance; mgrB.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / pharmacology
  • Anti-Bacterial Agents / therapeutic use
  • Bacterial Proteins / genetics
  • Brazil
  • Carbapenems / pharmacology
  • Colistin / pharmacology
  • DNA Transposable Elements
  • Drug Resistance, Bacterial
  • Humans
  • Klebsiella Infections* / drug therapy
  • Klebsiella pneumoniae* / genetics
  • Microbial Sensitivity Tests

Substances

  • Anti-Bacterial Agents
  • Bacterial Proteins
  • Carbapenems
  • DNA Transposable Elements
  • Colistin