Rapid and comprehensive profiling of α-glucosidase inhibitors in Buddleja Flos by ultrafiltration HPLC-QTOF-MS/MS with diagnostic ions filtering strategy

Lianwu Xie; Qiachi Fu; Shuyun Shi; Jiawei Li; Xinji Zhou

doi:10.1016/j.foodchem.2020.128651

Rapid and comprehensive profiling of α-glucosidase inhibitors in Buddleja Flos by ultrafiltration HPLC-QTOF-MS/MS with diagnostic ions filtering strategy

Food Chem. 2021 May 15:344:128651. doi: 10.1016/j.foodchem.2020.128651. Epub 2020 Nov 17.

Authors

Lianwu Xie¹, Qiachi Fu², Shuyun Shi³, Jiawei Li⁴, Xinji Zhou⁴

Affiliations

¹ College of Sciences, Central South University of Forestry and Technology, Changsha 410004, PR China. Electronic address: xielianwu@csuft.edu.cn.
² College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, PR China.
³ College of Sciences, Central South University of Forestry and Technology, Changsha 410004, PR China; College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, PR China. Electronic address: shuyshi@gmail.com.
⁴ College of Sciences, Central South University of Forestry and Technology, Changsha 410004, PR China.

PMID: 33243557
DOI: 10.1016/j.foodchem.2020.128651

Abstract

Buddleja Flos is used as yellow rice colorant and a well-known traditional Chinese medicine. But its biochemical profiling is still lack due to complex matrix. Here, ultrafiltration high-performance liquid chromatograph-quadrupole time-of-flight tandem mass spectrometry (HPLC-QTOF-MS/MS) with diagnostic ions filtering strategy was proposed for rapid and comprehensive investigation of its α-glucosidase inhibitors. As a result, 33 bioactive compounds (13 phenylethanoid glycosides and 20 flavonoids) were successfully screened and identified. In addition, α-glucosidase inhibitory activities of twenty-two references were verified. Six flavonoid aglycones (4, 28, and 30-33) showed excellent α-glucosidase inhibitory activities (IC₅₀, from 5.11 ± 0.85 to 32.49 ± 9.76 μg/mL), much higher than that of acarbose (IC₅₀, 195.49 ± 10.05 μg/mL). Five flavonoid-monoglycosides (7, 12, 13, 20, and 22) presented moderate inhibitory activities with IC₅₀ from 160.98 ± 23.19 to 249.37 ± 35.83 μg/mL. Results showcased the high efficiency of proposed strategy in profiling of bioactive compounds from natural products.

Keywords: Diagnostic ions filtering; Flavonoid; HPLC–QTOF-MS/MS; Phenylethanoid glycoside; Ultrafiltration; α-Glucosidase inhibitor.

MeSH terms

Buddleja / chemistry*
Chromatography, High Pressure Liquid / methods*
Glycoside Hydrolase Inhibitors / analysis*
Glycoside Hydrolase Inhibitors / isolation & purification*
Tandem Mass Spectrometry / methods*
Ultrafiltration / methods*

Substances

Glycoside Hydrolase Inhibitors