Feasibility of T1 mapping with histogram analysis for the diagnosis and staging of liver fibrosis: Preclinical results

Abstract

Purpose: To compare the diagnostic accuracy of parameters derived from the histogram analysis of precontrast, 10-min hepatobiliary phase (HBP) and 20-min HBP T1 maps for staging liver fibrosis (LF).

Methods: LF was induced in New Zealand white rabbits by subcutaneous injections of carbon tetrachloride for 4-16 weeks (n = 120), and 20 rabbits injected with saline served as controls. Precontrast, 10-min and 20-min HBP modified Look-Locker inversion recovery (MOLLI) T1 mapping was performed. Histogram analysis of T1 maps was performed, and the mean, median, skewness, kurtosis, entropy, inhomogeneity and 10th/25th/75th/90th percentiles of T1_native, T1_10min and T1_20min were derived. Quantitative histogram parameters were compared. For significant parameters, further receiver operating characteristic (ROC) analyses were performed to evaluate the potential diagnostic performance in differentiating LF stages.

Results: Finally, 17, 20, 21, 21 and 20 rabbits were included for the F0, F1, F2, F3, and F4 pathological grades of fibrosis, respectively. The mean/75th of T1_native, entropy of T1_10min and entropy/mean/median/10th of T1_20min demonstrated a significant good correlation with the LF stage (|r| = 0.543-0.866, all P < 0.05). The 75th of T1_native, entropy_10min, and entropy_20min were the three most reliable imaging markers in reflecting the stage of LF. The area under the ROC curve of entropy_20min was larger than that of entropy_10min (P < 0.05 for LF ≥ F2, ≥F3, and ≥ F4) and the 75th of T1_native (P < 0.05 for LF ≥ F2 and ≥ F3) for staging LF.

Conclusion: Magnetic resonance histogram analysis of T1 maps, particularly the entropy derived from 20-min HBP T1 mapping, is promising for predicting the LF stage.

Keywords: Histogram analysis; Liver fibrosis; T1 mapping.