Indole alkaloids from Ophiorrhiza cantoniensis with immunosuppressive activity

Fitoterapia. 2021 Jan:148:104777. doi: 10.1016/j.fitote.2020.104777. Epub 2020 Nov 22.

Abstract

Three new indole alkaloids, ophiorrhines C-D (1-3), together with one known analogue, have been isolated from the plant of Ophiorrhiza cantoniensis Hace. The structures of the new alkaloids with the absolute configurations were elucidated by means of spectroscopic methods, electronic circular dichroism (ECD) and calculated nuclear magnetic resonance (NMR) with DP4+ analysis. Compounds 1 and 2 exhibited certain activity to Con-A induced T cell proliferation, and 1 exhibited good inhibition on LPS-induced B cell proliferation with an IC50 value of 8.7 μM.

Keywords: Immunosuppressive activity; Indole alkaloids; Isolation and structural elucidation; Ophiorrhiza cantoniensis.

MeSH terms

  • Animals
  • B-Lymphocytes / drug effects
  • Cell Proliferation / drug effects
  • Cells, Cultured
  • China
  • Female
  • Immunosuppressive Agents / isolation & purification
  • Immunosuppressive Agents / pharmacology*
  • Indole Alkaloids / isolation & purification
  • Indole Alkaloids / pharmacology*
  • Mice
  • Mice, Inbred BALB C
  • Molecular Structure
  • Phytochemicals / isolation & purification
  • Phytochemicals / pharmacology
  • Rubiaceae / chemistry*
  • Spleen / cytology
  • T-Lymphocytes / drug effects

Substances

  • Immunosuppressive Agents
  • Indole Alkaloids
  • Phytochemicals