LncRNA-IUR Sponges miR-24 to Upregulate P53 in Laryngeal Squamous Cell Carcinoma

Cen Wei; Huaqing Wei; Xun Wu; Guangyao Nong; Chenglin Wu; Jinli Lee; Ning Meng; Dahai Yu; Jiping Su; Mengzhu Guo; Jiangyuan Qin; Xuemin Fan

doi:10.2147/CMAR.S236188

LncRNA-IUR Sponges miR-24 to Upregulate P53 in Laryngeal Squamous Cell Carcinoma

Cancer Manag Res. 2020 Nov 16:12:11639-11647. doi: 10.2147/CMAR.S236188. eCollection 2020.

Authors

Cen Wei^#¹, Huaqing Wei^#², Xun Wu³, Guangyao Nong¹, Chenglin Wu⁴, Jinli Lee⁵, Ning Meng³, Dahai Yu⁶, Jiping Su⁷, Mengzhu Guo⁶, Jiangyuan Qin⁸, Xuemin Fan³

Affiliations

¹ ENT & HN Surgery Department, Wuming Hospital of Guangxi Medical University, Nanning 530199, People's Republic of China.
² Department of Cardiology, Wuming Hospital of Guangxi Medical University, Nanning 530199, People's Republic of China.
³ Department of Maxillofacial Surgery, Guangxi Medical University College of Stomatology, Nanning 530021, People's Republic of China.
⁴ Department of ENT, Sixth Affiliated Hospital of Guangxi Medical University, Nanning 537000, People's Republic of China.
⁵ Department of Gastroenterology, 923 Hospital of the Joint Logistics Support Force of the Chinese People's Liberation Army, Nanning 530021, People's Republic of China.
⁶ Department of Stomatology, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, People's Republic of China.
⁷ ENT & HN Surgery Department, The First Affiliated Hospital of Guangxi Medical University, Nanning 530199, People's Republic of China.
⁸ Department of Otolaryngology, Guangxi General Hospital of Chinses People's Armed Police Force, Nanning 530000, People's Republic of China.

^# Contributed equally.

Abstract

Objective: The functions of lncRNA-IUR in laryngeal squamous cell carcinoma (LSCC) were investigated in this study.

Methods: RT-qPCR and paired t-test were used to measure and compare expression levels of IUR, miR-24 and p53 in LSCC and non-tumor tissues. Human LSCC cell line UM-SCC-17A was used and transfected by pcDNA3.1 vector to overexpress IUR and miR-24. The transwell assay and wound healing assay illustrated the effect of overexpression of IUR or miR-24 in the cell invasion and migration of LSCC. Subcutaneous tumor model in nude mice was carried out to demonstrate the mechanism between IUR and miR-24 in regulating tumor growth.

Results: We found that IUR was downregulated in LSCC. Low expression levels of IUR were correlated with the poor survival of LSCC patients. Overexpression experiments showed that overexpression of IUR led to increased, while overexpression of miR-24 led to decreased expression levels of p53 in LSCC cells. And bioinformatics analysis showed that IUR may sponge miR-24. Cell proliferation assay showed that overexpression of IUR and p53 led to decreased proliferation rate of LSCC cells, while overexpression of miR-24 led to increased proliferation rate of LSCC cells. We also illustrated that overexpression of IUR promoted cell migration and invasion while miR-24 had opposite effects. In addition, subcutaneous tumor model in nude mice showed that overexpression of miR-24 attenuated the effects of overexpression of IUR on the expression of p53 and cancer cell proliferation.

Conclusion: IUR sponges miR-24 to upregulate p53 in LSCC, thereby inhibiting cancer cell proliferation.

Keywords: IUR; laryngeal squamous cell carcinoma; miR-24; p53.

Grants and funding

This work was supported by Project of Clinical Research Foundation of Western Stomatology Chinese Stomatological Association (grant number CSA-W2018-06) & National Natural Science Foundation of China (grant number 81360407).