A multifunctional CeO2@SiO2-PEG nanoparticle carrier for delivery of food derived proanthocyanidin and curcumin as effective antioxidant, neuroprotective and anticancer agent

Zhongqin Chen; Leilei Xu; Xudong Gao; Chunli Wang; Ruilin Li; Jun Xu; Min Zhang; Pharkphoom Panichayupakaranant; Haixia Chen

doi:10.1016/j.foodres.2020.109674

A multifunctional CeO₂@SiO₂-PEG nanoparticle carrier for delivery of food derived proanthocyanidin and curcumin as effective antioxidant, neuroprotective and anticancer agent

Food Res Int. 2020 Nov:137:109674. doi: 10.1016/j.foodres.2020.109674. Epub 2020 Sep 17.

Authors

Zhongqin Chen¹, Leilei Xu¹, Xudong Gao¹, Chunli Wang¹, Ruilin Li¹, Jun Xu¹, Min Zhang², Pharkphoom Panichayupakaranant³, Haixia Chen⁴

Affiliations

¹ Tianjin Key Laboratory for Modern Drug Delivery & High-Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, PR China.
² Tianjin Agricultural University, Tianjin 300384, PR China; State Key Laboratory of Nutrition and Safety, Tianjin University of Science & Technology, Tianjin 300457, PR China.
³ Phytomedicine and Pharmaceutical Biotechnology Excellence Center, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat-Yai, Songkhla 90112, Thailand.
⁴ Tianjin Key Laboratory for Modern Drug Delivery & High-Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, PR China. Electronic address: chenhx@tju.edu.cn.

PMID: 33233251
DOI: 10.1016/j.foodres.2020.109674

Abstract

The nanoparticle systems could effectively overcome the drug delivery challenges of food bioactive compounds. In this study, a novel and effective multifunctional PEG modified CeO₂@SiO₂ nanoparticle (CSP-NPs) system was successfully fabricated. Food derived proanthocyanidin (PAC) and curcumin (Cur) were loaded onto CSP-NPs and formed as PAC-NPs and Cur-NPs. Fourier transform Infrared spectra, X-ray diffraction, scanning electron microscopy, transmission electron microscopy, and dynamic light scattering were used to characterize the prepared NPs. CSP-NPs, PAC-NPs and Cur-NPs displayed spherical shape with about 35-45 nm size. The bioactivity analysis revealed that CSP-NPs system could effectively deliver PAC and Cur to exhibit strong antioxidant activity, potent neuroprotective effect against Aβ_1-42-mediated toxicity in PC-12 cells (recovered cell viability from 57.5% to 81.0% at the dose of 25 μg/mL) and effective antiproliferative effects on HepG2 and Hela cells. Besides, all prepared nanoparticles (0-100 µg/ml) used in this study showed no significant toxicity on cell models of antioxidative and neuroprotective activities, excepting for cancer cells, suggesting that these nanoparticles had the potential of being utilized in drug delivery. Therefore, CSP-NPs might be a promising delivery system for hydrophilic molecule proanthocyanidin and hydrophobic molecule curcumin against the oxidative damage, neurodegenerative diseases and cancer, which could facilitate the application of food derived nutrients in functional foods industry.

Keywords: Acetylthiocholine Iodide (PubChem CID: 74629); Ammonia aqueous (PubChem CID: 14923); Ammonium Carbonate (PubChem CID: 24660); Anticancer activity; Antioxidant activity; Cerium Nitrate Hexahydrate (PubChem CID: 16211466); Cerium oxide (PubChem CID: 73963); Curcumin; Curcumin (PubChem CID: 969516); Multifunctional nanoparticle; Neuroprotective effect; Polyethylene glycol 6000 (PubChem CID: 174); Proanthocyanidin; Proanthocyanidin B5 (PubChem CID: 124017); Silicon dioxide (PubChem CID: 24261); Sodium Dodecyl Benzene Sulfonate (PubChem CID: 23662403).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antineoplastic Agents*
Antioxidants / pharmacology
Curcumin* / pharmacology
Drug Carriers
HeLa Cells
Humans
Nanoparticles*
Proanthocyanidins*
Silicon Dioxide

Substances

Antineoplastic Agents
Antioxidants
Drug Carriers
Proanthocyanidins
Silicon Dioxide
Curcumin