A novel sustainable approach for the extraction of value-added compounds from Hibiscus sabdariffa L. calyces by natural deep eutectic solvents

M Elena Alañón; Milena Ivanović; Sandra Pimentel-Mora; Isabel Borrás-Linares; David Arráez-Román; Antonio Segura-Carretero

doi:10.1016/j.foodres.2020.109646

A novel sustainable approach for the extraction of value-added compounds from Hibiscus sabdariffa L. calyces by natural deep eutectic solvents

Food Res Int. 2020 Nov:137:109646. doi: 10.1016/j.foodres.2020.109646. Epub 2020 Sep 1.

Authors

M Elena Alañón¹, Milena Ivanović², Sandra Pimentel-Mora³, Isabel Borrás-Linares⁴, David Arráez-Román³, Antonio Segura-Carretero³

Affiliations

¹ Area of Food Science and Technology, Department of Analytical Chemistry and Food Science, Higher Technical School of Agronomic Engineering, University of Castilla-La Mancha, Ronda de Calatrava 7, 13071 Ciudad Real, Spain; Department of Analytical Chemistry, Faculty of Sciences, University of Granada, C/Fuentenueva s/n, 18071 Granada, Spain; Research and Development of Functional Food Centre (CIDAF), PTS Granada, Avda. Del Conocimiento 37, Bioregión Building, 18016 Granada, Spain. Electronic address: mariaelena.alanon@uclm.es.
² Faculty of Chemistry and Chemical Engineering, University of Maribor, Smetanova ulica 17, SI-2000 Maribor, Slovenia.
³ Department of Analytical Chemistry, Faculty of Sciences, University of Granada, C/Fuentenueva s/n, 18071 Granada, Spain; Research and Development of Functional Food Centre (CIDAF), PTS Granada, Avda. Del Conocimiento 37, Bioregión Building, 18016 Granada, Spain.
⁴ Research and Development of Functional Food Centre (CIDAF), PTS Granada, Avda. Del Conocimiento 37, Bioregión Building, 18016 Granada, Spain.

PMID: 33233225
DOI: 10.1016/j.foodres.2020.109646

Abstract

Hibiscus sabdariffa L. is widely acknowledged as an important source of value-added compounds as natural pigments and bioactive compounds whose isolation is of great interest in food industry. Due to the incessant demand of green extraction procedures, NADESs have emerged in the last years as new green solvents alternative to the conventional organic ones. The feasibility of NADESs for the extraction of value-added compounds of H. sabdariffa has been assessed in this work for the first time. An extraction technique based on microwave assisted extraction (MAE) and the use of NADESs was proposed testing different deep eutectic solvents based on choline chloride (ChCl). Among them, oxalic acid based NADES exhibited a great selectivity for anthocyanins in particular as well as higher extraction yields of bioactive compounds was also observed, even greater than those observed by methanol. The effect of extraction variables such as liquid-solid ratio, temperature and water percentage were studied by Box-Behnken design and a response surface methodology. The statistical program pointed out the liquid-solid ratio of 1:30 mL mg^-1, temperature of 75 °C and a water percentage of 55% as the conditions to maximize extraction within the experimental domain. Hence, NADES composed by oxalic acid and choline chloride resulted to be an effective green alternative tailor-made solvent to carry out selective extractions of value-added compounds from H. sabdariffa.

Keywords: Green extraction; Hibiscus sabdariffa L.; Microwave-assisted extraction; Natural deep eutectic solvents (NADES); Value-added compounds.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anthocyanins
Hibiscus*
Methanol
Plant Extracts
Solvents

Substances

Anthocyanins
Plant Extracts
Solvents
Methanol