LC-MS/MS screening and identification of bioactive compounds in leaves, pulp and seed from Eugenia calycina Cambess

Nayara Macêdo Peixoto Araujo; Henrique Silvano Arruda; Fábio Neves Dos Santos; Damila Rodrigues de Morais; Gustavo Araujo Pereira; Glaucia Maria Pastore

doi:10.1016/j.foodres.2020.109556

LC-MS/MS screening and identification of bioactive compounds in leaves, pulp and seed from Eugenia calycina Cambess

Food Res Int. 2020 Nov:137:109556. doi: 10.1016/j.foodres.2020.109556. Epub 2020 Jul 16.

Authors

Nayara Macêdo Peixoto Araujo¹, Henrique Silvano Arruda², Fábio Neves Dos Santos³, Damila Rodrigues de Morais⁴, Gustavo Araujo Pereira⁵, Glaucia Maria Pastore⁶

Affiliations

¹ Bioflavors and Bioactive Compounds Laboratory, Department of Food Science, School of Food Engineering, University of Campinas (UNICAMP), 13083-862 Campinas, SP, Brazil. Electronic address: nayarapeixoto14@hotmail.com.
² Bioflavors and Bioactive Compounds Laboratory, Department of Food Science, School of Food Engineering, University of Campinas (UNICAMP), 13083-862 Campinas, SP, Brazil; Nutrition and Metabolism Laboratory, Department of Food and Nutrition, School of Food Engineering, University of Campinas (UNICAMP), 13083-862 Campinas, SP, Brazil.
³ Thomson Mass Spectrometry Laboratory, Institute of Chemistry, University of Campinas (UNICAMP), 13083-970 Campinas, SP, Brazil.
⁴ Shimadzu do Brasil Comércio Ltda., 06455-000 Barueri, SP, Brazil.
⁵ School of Food Engineering (FEA), Institute of Technology (ITEC), Federal University of Pará (UFPA), 66075-110 Belém, PA, Brazil.
⁶ Bioflavors and Bioactive Compounds Laboratory, Department of Food Science, School of Food Engineering, University of Campinas (UNICAMP), 13083-862 Campinas, SP, Brazil.

PMID: 33233178
DOI: 10.1016/j.foodres.2020.109556

Abstract

The Eugenia calycina Cambess, also known as pitanga-do-cerrado, is an unexplored Brazilian fruit used by native people for the treatment of diabetes. The aim of this study was to identify the phenolic compounds from the leaves, pulp and seed of Eugenia calycina by using LC-MS-based targeted and untargeted analysis. The LC-MS-based targeted quantitative analysis showed a high phenolic content in all plant parts in which the ellagic acid was the main phenolic compound with values of 8244.53 µg/g dw (leaves), 5054.43 µg/g dw (pulp) and 715.42 µg/g dw (seed). The leaves, pulp and seed showed a high total phenolic content of 20371.96, 7139.70 and 2204.75 µg/g dw, respectively. In addition, the LC-MS-based targeted analysis showed ellagic acid, myricitrin and epicatechin gallate as the main phenolic compounds in the Eugenia calycina. The LC-MS-based untargeted analysis showed the phytochemical profile of the leaves, pulp and seed in which 153 phytochemicals from different chemical classes were annotated, including organic acids, phenolic acids, flavonoids, and other compounds. The Eugenia calycina has high potential as a plant-based food due to its phytochemical profile and high phenolic content.

Keywords: Eugenia species; Flavonoids; Phenolic acids; Phytochemical composition; Pitanga-do-cerrado.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Brazil
Chromatography, Liquid
Eugenia*
Humans
Plant Extracts
Plant Leaves
Seeds
Tandem Mass Spectrometry

Substances

Plant Extracts