Identification of a New Chromosomal Region Involved in the Genetic Control of Resistance to Anthracnose in Common Bean

Abstract

Anthracnose caused by Colletotrichum lindemuthianum (Sacc. & Magnus) Lams.-Scrib. is a major disease affecting common bean (Phaseolus vulgaris L.) crops worldwide. Response to five C. lindemuthianum isolates, classified as races 3, 6, 7, 38, and 73, were analyzed in 156 F_2:3 families derived from the cross between line SEL1308 and cultivar Michigan Dark Red Kidney (MDRK). SEL1308 was resistant to all five races, while MDRK was susceptible to all except for race 73. Segregation ratio for response to races 3 and 7 indicated that single dominant genes were responsible for the resistance reaction to each race. Recombination between both race-specific genes was observed and no linkage was found with any of the molecular markers tagging Co-genes or clusters previously described. Linkage analyses allowed the location of both genes at the beginning of linkage group (LG) Pv03, a region tentatively named as Co-17. Segregation ratio for response to races 6 and 38 indicated that two dominant and independent genes conferred resistance to these races. Contingency tests and subpopulation analyses suggested the implication of one region on LG Pv08, corresponding to the Co-4 cluster, and the Co-17 region. For reaction to race 73, the most likely scenario was that two dominant and independent genes conferred resistance: Co-1 in MDRK and Co-4² in SEL1308. Results indicated that, in addition to Co-4² , SEL1308 carries resistance genes located at the beginning of LG Pv03, in which no anthracnose resistance genes were previously mapped. In silico analysis revealed the presence of seven genes codifying typical resistance proteins (R-proteins) in the underlying physical position of the Co-17 region.