Surface Acoustic Wave Sensor for C-Reactive Protein Detection

Ming-Jer Jeng; Mukta Sharma; Ying-Chang Li; Yi-Chen Lu; Chia-Yu Yu; Chia-Lung Tsai; Shiang-Fu Huang; Liann-Be Chang; Chao-Sung Lai

doi:10.3390/s20226640

Surface Acoustic Wave Sensor for C-Reactive Protein Detection

Sensors (Basel). 2020 Nov 19;20(22):6640. doi: 10.3390/s20226640.

Authors

Ming-Jer Jeng^{1

2}, Mukta Sharma¹, Ying-Chang Li³, Yi-Chen Lu¹, Chia-Yu Yu¹, Chia-Lung Tsai^{1

2}, Shiang-Fu Huang^{2

4}, Liann-Be Chang^{1

2}, Chao-Sung Lai¹

Affiliations

¹ Department of Electronic Engineering, Chang Gung University, Taoyuan 333, Taiwan.
² Department of Otolaryngology-Head and Neck Surgery, Chang Gung Memorial Hospital, Linkou 244, Taiwan.
³ Green Technology Research Center, Chang Gung University, Taoyuan 333, Taiwan.
⁴ Department of Public Health, Chang Gung University, Taoyuan 333, Taiwan.

Abstract

A surface acoustic wave (SAW) sensor was investigated for its application in C-reactive protein (CRP) detection. Piezoelectric lithium niobate (LiNbO₃) substrates were used to study their frequency response characteristics in a SAW sensor with a CRP sensing area. After the fabrication of the SAW sensor, the immobilization process was performed for CRP/anti-CRP interaction. The CRP/anti-CRP interaction can be detected as mass variations in the sensing area. These mass variations may produce changes in the amplitude of sensor response. It was clearly observed that a CRP concentration of 0.1 μg/mL can be detected in the proposed SAW sensor. A good fitting linear relationship between the detected insertion loss (amplitude) and the concentrations of CRP from 0.1 μg/mL to 1 mg/mL was obtained. The detected shifts in the amplitude of insertion loss in SAW sensors for different CRP concentrations may be useful in the diagnosis of risk of cardiovascular diseases.

Keywords: C-reactive protein; piezoelectric lithium niobate; surface acoustic wave.

Publication types

Letter

MeSH terms

C-Reactive Protein* / analysis
Cardiovascular Diseases* / diagnosis
Sound*

Substances

C-Reactive Protein