Cloning of Monkeys by Somatic Cell Nuclear Transfer

Shiyu Sun; Zhaodi Liao; Zhen Liu; Qiang Sun

doi:10.1007/978-1-0716-1084-8_2

Cloning of Monkeys by Somatic Cell Nuclear Transfer

Methods Mol Biol. 2021:2239:19-31. doi: 10.1007/978-1-0716-1084-8_2.

Authors

Shiyu Sun¹, Zhaodi Liao¹, Zhen Liu¹, Qiang Sun²

Affiliations

¹ Institute of Neuroscience, State Key Laboratory of Neuroscience, CAS Key Laboratory of Primate Neurobiology, CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai, China.
² Institute of Neuroscience, State Key Laboratory of Neuroscience, CAS Key Laboratory of Primate Neurobiology, CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai, China. qsun@ion.ac.cn.

PMID: 33226610
DOI: 10.1007/978-1-0716-1084-8_2

Abstract

Somatic cell nuclear transfer (SCNT) is a promising method to establish genetically modified monkeys with identical genetic background as models in biomedical research. We have recently cloned monkeys by optimization of the SCNT protocols and inclusion of the epigenetic modulator. Here, we describe the protocol for generation of cloned monkeys by somatic cell nuclear transfer.

Keywords: Genetically modified monkey models; Monkey cloning; Somatic cell nuclear transfer.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Animals, Genetically Modified
Cell Culture Techniques / methods
Cells, Cultured
Cloning, Organism / instrumentation
Cloning, Organism / methods*
Embryo, Mammalian / metabolism
Embryo, Mammalian / physiology
Epigenesis, Genetic
Female
Fibroblasts / cytology*
Haplorhini / genetics*
Microsatellite Repeats
Nuclear Transfer Techniques* / instrumentation
Oocytes / cytology*
Polymorphism, Single Nucleotide
Pregnancy
Ultrasonography