To understand the relationship between the community structure of bacteria and soil environment, the diversity and composition of soil bacterial communities were investigated, based on 16S rRNA gene clone library, in three different sampling sites (SP1, SP2 and SP3) in the Bortala and Jinghe River basins of Ebinur Lake Wetland. The results showed that the diversity of bacteria among plots was SP2 > SP3 > SP1, and the richness was SP3 > SP2 > SP1. Community composition analysis of bacteria showed that Proteobacteria and Bacteroides accounted for 49.7% and 53.7%, respectively, making them the most dominant phyla observed. In SP1, Proteobacteria was the most dominant phylum, followed by Bacteroides. In SP2 and SP3, Bacteroides was the most dominant phylum, followed by Proteobacteria. At subphyla level, Gammaproteobacteria, Alphaproteobacteria and Deltaproteobacteria accounted for 50%, 51.0%, and 42.2% of the Proteobacteria of SP1, SP2, and SP3, respectively. Betaproteobacteria and Epsilonproteobacteria were found only in SP3. RDA results showed that SOM, SM and EC were the main soil environmental factors affecting bacterial community structure.
Keywords: 16S rRNA gene clone library; Community structure; Diversity and richness; RDA analysis; The Ebinur Lake Wetland.