Multiple Components Rapidly Screened from Perilla Leaves Attenuate Asthma Airway Inflammation by Synergistic Targeting on Syk

Hui Yang; Wei Sun; Pei Ma; Chunsuo Yao; Yannan Fan; Shuyi Li; Jiqiao Yuan; Ziqian Zhang; Xuyu Li; Mingbao Lin; Qi Hou

doi:10.2147/JIR.S281393

Multiple Components Rapidly Screened from Perilla Leaves Attenuate Asthma Airway Inflammation by Synergistic Targeting on Syk

J Inflamm Res. 2020 Nov 13:13:897-911. doi: 10.2147/JIR.S281393. eCollection 2020.

Authors

Hui Yang¹, Wei Sun², Pei Ma¹, Chunsuo Yao¹, Yannan Fan¹, Shuyi Li¹, Jiqiao Yuan¹, Ziqian Zhang¹, Xuyu Li¹, Mingbao Lin¹, Qi Hou¹

Affiliations

¹ State Key Laboratory of Bioactive Substances and Functions of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, People's Republic of China.
² Beijing Friendship Hospital, Capital Medical University, Beijing, People's Republic of China.

Abstract

Background: Perilla frutescens (L.) Britt., a classic medicinal plant, has been demonstrated to have anti-inflammatory and anti-allergic effects in asthma. Perilla leaves extract (PLE) exerted significant therapeutic effect against allergic asthma inflammation through Syk inhibition. But the active chemical ingredients from PLE are complex and unclear, it is difficult to fully elucidate its pharmacological mechanisms.

Methods: A method was established for rapid screening and characterization of active ingredients from PLE that targeted Syk, with which three potential active ingredients were identified. By using OVA-induced allergic asthma mouse model in vivo, OVA-induced human PBMCs inflammation model and DNP-IgE/BSA-induced RBL-2H3 cells model in vitro, the effects and mechanisms of PLE and its active components were evaluated.

Results: Using Syk-affinity screening method, roseoside (RosS), vicenin-2 (Vic-2) and rosmarinic acid (RosA) were identified from PLE. In vitro, PLE and its ingredients showed significant inhibitory activities against Syk, with their mixture (Mix, prepared by RosS, Vic-2 and RosA in accordance with their ratio in Syk-conjugated beads bound fraction) showing a stronger inhibitory activity. RosS, Vic-2 and RosA also showed significant effects on allergic asthma, and a synergistic effect of Mix was observed. Moreover, treatment with PLE, RosS, Vic-2, RosA, and Mix significantly inhibited the expression and phosphorylation of Syk, PKC, NF-κB p65, and cPLA₂ in allergic mice lung tissue and in RBL-2H3 cells.

Conclusion: PLE may alleviate allergic airway inflammation partly through the multiple components synergistic targeting on Syk and its downstream inflammatory pathway.

Keywords: PLE; Syk; allergic airway inflammation; multi-component; synergistic effect.

Grants and funding

This work was financially supported by the National Natural Science Foundation of China (Grant No. 81473398, 81973539 and 81803810), the Beijing Natural Science Foundation Program (Grant no. 7182116), the CAMS Initiative for Innovative Medicine (Grant no. 2016-I2M-2-006), the Drug Innovation Major Project of China (Grant no. 2018ZX09711001-003-001), the PUMC Graduate Innovation Fund (Grant no. 2019-1007-14), and the Funds of State Key Laboratory of Bioactive Substance and Function of Natural Medicines of China (Grant No. GTZK201807). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.