Preparation and characterization of pH-responsive microgel using arabinoxylan from wheat bran for BSA delivery

Juan Li; Zhenjie Lu; Zhengxing Chen; Cheng Li; Yan Du; Chen Chen; Li Wang; Peibin Yu

doi:10.1016/j.foodchem.2020.128220

Preparation and characterization of pH-responsive microgel using arabinoxylan from wheat bran for BSA delivery

Food Chem. 2021 Apr 16:342:128220. doi: 10.1016/j.foodchem.2020.128220. Epub 2020 Oct 8.

Authors

Juan Li¹, Zhenjie Lu¹, Zhengxing Chen², Cheng Li¹, Yan Du¹, Chen Chen¹, Li Wang¹, Peibin Yu¹

Affiliations

¹ The Key Laboratory of Synthetic and Biological Colloids, Ministry of Education, Jiangnan University, Wuxi, Jiangsu 214122, China; National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi, Jiangsu 214122, China; Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, Jiangnan University, Wuxi 214122, China; Jiangsu Provincial Research Center for Bioactive Product Processing Technology, Jiangnan University, Wuxi 214122, China.
² The Key Laboratory of Synthetic and Biological Colloids, Ministry of Education, Jiangnan University, Wuxi, Jiangsu 214122, China; National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi, Jiangsu 214122, China; Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, Jiangnan University, Wuxi 214122, China; Jiangsu Provincial Research Center for Bioactive Product Processing Technology, Jiangnan University, Wuxi 214122, China. Electronic address: zxchen2019@126.com.

PMID: 33223291
DOI: 10.1016/j.foodchem.2020.128220

Abstract

Wheat bran arabinoxylan (AX) discard from wheat production was utilized to form pH-responsive microgels. AX was modified by carboxymethylation, and the carboxymethylated arabinoxylans (CMAX) were characterized by FT-IR, NMR, gel permeation chromatography (GPC), and rheological analysis. The CMAX microgel was cross-linked by Fe³⁺ using an inverse emulsification polymerization. The morphology, particle size, pH sensitivity, and mechanism of cross-linking between COO^- and Fe³⁺ of the CMAX microgel was investigated. The CMAX microgel was used to be an oral protein drug carrier. The CMAX microgel particles exhibited a stable spherical structure. FT-IR spectral analysis of the CMAX microgel indicated that the microgel was crosslinked by bridging Fe³⁺ and COO^- with unidentate binding. The CMAX microgel exhibited good pH sensitivity and high stability in acid condition. Additionally, BSA was used as the embedding protein, and the controlled release effect of CMAX microgel was explored in gastrointestinal tract simulation.

Keywords: Arabinoxylan; Carboxymethylation; Controlled release; Inverse emulsification polymerization; Microgel; Protein delivery; pH-responsive.

MeSH terms

Animals
Cattle
Dietary Fiber / analysis*
Drug Carriers / chemistry*
Hydrogen-Ion Concentration
Microgels / chemistry*
Particle Size
Serum Albumin, Bovine / chemistry*
Xylans / chemistry*

Substances

Dietary Fiber
Drug Carriers
Microgels
Xylans
Serum Albumin, Bovine
arabinoxylan