Expression of lysophosphatidic acid receptor 1 in the adult female mouse pituitary gland

Neurosci Lett. 2021 Jan 10:741:135506. doi: 10.1016/j.neulet.2020.135506. Epub 2020 Nov 19.

Abstract

Lysophosphatidic acid receptor 1 (LPA1) is a receptor of lysophosphatidic acid (LPA). The present study investigated Lpar1 mRNA expression in the mouse pituitary gland by RT-PCR, in situ hybridization, and immunohistochemistry. Lpar1 mRNA was abundantly expressed in the pituitary gland. In situ hybridization and immunohistochemistry revealed over 90 % of a common glycoprotein α-subunit, luteinizing hormone β-subunit, and thyroid-stimulating hormone β-subunit immunoreactive cells co-expressed Lpar1 mRNA in the anterior pituitary gland, but few growth hormone, adrenocorticotropic hormone, and prolactin cells co-expressed Lpar1. Furthermore, Lpar1 mRNA levels in the pituitary gland were increased after ovariectomy and decreased after E2 administration. These results demonstrate that LPA1-mediated signaling may play physiological roles in gonadotropes and thyrotropes in the mouse pituitary gland.

Keywords: Estrogen; Gonadotrope; LPA(1); Lysophosphatidic acid; Pituitary; Thyrotrope.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Estrogens / administration & dosage
  • Estrous Cycle / metabolism
  • Female
  • Gene Expression
  • Mice
  • Mice, Inbred ICR
  • Pituitary Gland / drug effects
  • Pituitary Gland / metabolism*
  • Protein Subunits / metabolism
  • RNA, Messenger / metabolism
  • Receptors, Lysophosphatidic Acid / metabolism*

Substances

  • Estrogens
  • Protein Subunits
  • RNA, Messenger
  • Receptors, Lysophosphatidic Acid
  • lysophosphatidic acid receptor 1, mouse