The aim of this paper is to investigate the effects of a rotating ion beam on the temperature gradient instability (TGI) in completely ionized plasmas. The interplay of the temperature and density gradients provides the basis for experiencing an unstable inhomogeneous plasma medium due to TGI taken under consideration. The density and temperature gradients are considered perpendicular to the magnetic field where a nonrelativistic rotating ion beam such as O^{+} is present. By implementing the kinetic theory together with a zeroth-order approximation of geometrical optics, the dielectric permittivity tensor of the inhomogeneous plasma is obtained where by a suitable linear eikonal equation, the growth rate of the TGI in the collisional regime is calculated in the presence of a rotating ion beam. In such a configuration an unstable condition is experienced in regions with opposite electron density and temperature gradients, where it is destabilized by the temperature and plasma density gradients and the frequent electron collisions. As a consequence, the results reveal that the TGI can be damped or modified through interaction with the rotating ion beam depending on the characteristics of the ion beam, namely, velocity and density.