The effect of the inclusion of pea in lamb fattening concentrate on in vitro and in situ rumen fermentation

J Sci Food Agric. 2021 May;101(7):3041-3048. doi: 10.1002/jsfa.10938. Epub 2020 Nov 29.

Abstract

Background: The objective of this study was to evaluate the effect of the proportion of pea (0%, 10%, 20%, and 30%), as a partial replacement for soybean meal (SBM) in the fattening concentrate, on ruminal fermentation in lambs. Gas and methane (CH4 ) production, in vitro dry matter degradability (IVDMD), ammonia (NH3 -N), and volatile fatty acid (VFA) production after 24 h of incubation were evaluated. The concentrates were also incubated in the rumen of the wethers for 2, 4, 6, 8, 12, and 24 h to evaluate the effects of pea inclusion on in situ dry matter degradability (DMD), organic matter degradability (OMD), nitrogen degradability (ND), NH3 -N, and VFA production.

Results: In the in vitro assay, the inclusion of pea only affected gas production (mL day-1 degraded dry matter), CH4 production (mL day-1 degraded dry matter), and IVDMD (P < 0.05), and tended to affect NH3 -N content (P < 0.10) without affecting VFA production. In the in situ assay, the inclusion of pea increased DMD, OMD, and ND linearly (P < 0.001), whereas pea inclusion decreased NH3 -N content linearly (P < 0.05). Neither total VFA production nor the proportion of acetic acid were affected by pea inclusion (P > 0.05), but the propionic proportion increased with the proportion of pea included.

Conclusions: The best level of pea inclusion in the concentrate could not be established based on the results of this study. However, the results showed that the inclusion of pea provides a good alternative protein source. © 2020 Society of Chemical Industry.

Keywords: Pisum sativum; concentrates; degradation; protein; sheep.

MeSH terms

  • Ammonia / metabolism
  • Animal Feed / analysis*
  • Animals
  • Digestion
  • Fatty Acids, Volatile / metabolism
  • Female
  • Fermentation
  • Male
  • Methane / metabolism
  • Pisum sativum / metabolism*
  • Rumen / metabolism*
  • Sheep / growth & development
  • Sheep / metabolism*

Substances

  • Fatty Acids, Volatile
  • Ammonia
  • Methane