Colorimetric Detection of Class A Soybean Saponins by G-Quadruplex-Based Hybridization Chain Reaction

Congcong Yin; Qiaoling Zhao; Aiqin Yue; Weijun Du; Dingbin Liu; Jinzhong Zhao; Yongpo Zhang; Min Wang

doi:10.1155/2020/8813239

Colorimetric Detection of Class A Soybean Saponins by G-Quadruplex-Based Hybridization Chain Reaction

J Anal Methods Chem. 2020 Nov 5:2020:8813239. doi: 10.1155/2020/8813239. eCollection 2020.

Authors

Congcong Yin¹, Qiaoling Zhao¹, Aiqin Yue², Weijun Du², Dingbin Liu³, Jinzhong Zhao¹, Yongpo Zhang¹, Min Wang²

Affiliations

¹ College of Arts and Sciences, Shanxi Agricultural University, Taigu 030801, Shanxi, China.
² College of Agronomy, Shanxi Agricultural University, Taigu 030801, Shanxi, China.
³ College of Chemistry, Nankai University, Tianjin 300071, China.

Abstract

Soybean saponin is one of the important secondary metabolites in seeds, which has various beneficial physiological functions to human health. GmSg-1 gene is the key enzyme gene for synthesizing class A saponins. It is of great significance to realize the visual and rapid detection of class A saponins at the genetic level. The hybridization chain reaction (HCR) was employed to the visual detection of GmSg-1 gene, which was implemented by changing the length of the target fragment to 92 bp and using the hairpin probes we designed to detect the GmSg-1 ^a and GmSg-1 ^b genes. The best condition of HCR reaction is hemin (1.2 μM), Triton X-100 (0.002%), ABTS (3.8 μM), and H₂O₂ (1.5 mM). It was found that HCR has high specificity for GmSg-1 gene and could be applied to the visual detection of different soybean cultivars containing Aa type, Ab type, and Aa/Ab type saponins, which could provide technical reference and theoretical basis for molecular breeding of soybean and development of functional soybean products.