First Full-Length Genome Sequence of Dengue Virus Serotype 2 Circulating in Vietnam in 2017

Thuy Thi Dang; My Ha Pham; Huy Vu Bui; Duyet Van Le

doi:10.2147/IDR.S275645

First Full-Length Genome Sequence of Dengue Virus Serotype 2 Circulating in Vietnam in 2017

Infect Drug Resist. 2020 Nov 10:13:4061-4068. doi: 10.2147/IDR.S275645. eCollection 2020.

Authors

Thuy Thi Dang¹, My Ha Pham², Huy Vu Bui^{1

3}, Duyet Van Le¹

Affiliations

¹ National Hospital for Tropical Diseases, Hanoi, Vietnam.
² Oxford University Clinical Research Unit, Hanoi, Vietnam.
³ Hanoi Medical University, Hanoi, Vietnam.

Abstract

Introduction: Dengue hemorrhagic fever is caused by four serotypes of dengue viruses transmitted by mosquitoes. In Vietnam, dengue outbreaks occur every year, and all four serotypes have been found circulating with the dominant one varying over time. However, in 2017 an unusual dengue fever outbreak occurred in the North of Vietnam, predominantly caused by DENV1 (92%) and DENV2 (7.3%). The objective of the present study was to obtain and characterize the full-length genome sequence of seven DENV2 strains in 2017 epidemic.

Materials and methods: Whole-genome sequencing of seven DENV2 isolates from the 2017 outbreak were obtained using the Illumina MiSeq next generation sequencer system. Complete genome sequences were then analyzed to find out genetic variants and genetic relationships between these DENV2 with other strains that circulated in Vietnam previously and other regions of the world.

Results: The complete genome sequence of seven DENV2 isolates in the 2017 dengue outbreak comprised 10,696 nucleotides with an open reading frame coding for 3392 amino acids. The genome analysis showed only a small number of amino acid changes which were obtained in all genes, in which a few amino acids substitutions were distributed over the positions such as G156 (NS1), V106 (NS2A), and L258/T260 (NS5). The phylogenetic analysis revealed that the DENV2 isolates in the 2017 outbreak were most closely related to the dengue virus from India in 2006, suggesting that the causative virus originated from the DENV2 that caused dengue hemorrhagic fever in 2006 in India.

Conclusion: The first complete genome sequences of seven DENV2 isolates in the 2017 dengue outbreak in Northern Vietnam were successfully obtained. The genetic and phylogenetic data indicated that these DENV2 isolates were not causative virus circulating in Vietnam previously but originated from India in 2006. These data are emerging and providing valuable information for the management and surveillance of dengue in Vietnam.

Keywords: DENV; DENV2; dengue epidemic; dengue virus; dengue virus type 2; full-length genome; genetic variants.

Grants and funding

This research was supported in part by a grant from the Ministry of Science and Technology of Vietnam (Grant number KC.10.22/16–20).