Low-dose Bisphenol-A Promotes Epigenetic Changes at Pparγ Promoter in Adipose Precursor Cells

Nutrients. 2020 Nov 13;12(11):3498. doi: 10.3390/nu12113498.

Abstract

Exposure to endocrine-disrupting chemicals such as Bisphenol-A (BPA) is associated with an increase in obesity prevalence. Diet is the primary cause of human exposure to this contaminant. BPA promotes obesity by inducing adipocyte dysfunction and altering adipogenesis. Contradictory evidence and unanswered questions are reported in the literature concerning the BPA effects on adipogenesis. To clarify this issue, we tested the effects of prolonged low-dose BPA exposure on different phases of adipogenesis in committed 3T3L1 and uncommitted NIH3T3 preadipocytes. Our findings show that BPA effects on the adipogenesis are mediated by epigenetic mechanisms by reducing peroxisome proliferator-activated receptor gamma (Pparγ) promoter methylation in preadipocytes. Nevertheless, in BPA-exposed 3T3L1, Pparγ expression only transiently increases as lipid accumulation at day 4 of differentiation, without altering the adipogenic potential of the precursor cells. In the absence of differentiation mix, BPA does not make the 3T3L1 an in vitro model of spontaneous adipogenesis and the effects on the Pparγ expression are still limited at day 4 of differentiation. Furthermore, BPA exposure does not commit the NIH3T3 to the adipocyte lineage, although Pparγ overexpression is more evident both in preadipocytes and during the adipocyte differentiation. Interestingly, termination of the BPA exposure restores the Pparγ promoter methylation and inflammatory profile of the 3T3L1 cells. This study shows that BPA induces epigenetic changes in a key adipogenic gene. These modifications are reversible and do not affect preadipocyte commitment and/or differentiation. We identify an alternative transcriptional mechanism by which BPA affects gene expression and demonstrate how the challenge of preventing exposure is fundamental for human health.

Keywords: Bisphenol-A; adipogenesis; adipose tissue; adipose tissue dysfunction; diabetes; environmental pollutant; food contaminant; inflammation; insulin resistance; obesity.

MeSH terms

  • 3T3-L1 Cells
  • Adipocytes / drug effects*
  • Adipogenesis / drug effects
  • Adipose Tissue / metabolism*
  • Animals
  • Benzhydryl Compounds / pharmacology*
  • Cell Differentiation
  • Endocrine Disruptors
  • Environmental Pollutants
  • Epigenesis, Genetic / drug effects*
  • Food Contamination
  • Gene Expression
  • Inflammation
  • Insulin Resistance
  • Mice
  • NIH 3T3 Cells
  • Obesity / metabolism
  • PPAR gamma / genetics*
  • PPAR gamma / metabolism*
  • Phenols / pharmacology*
  • Promoter Regions, Genetic*

Substances

  • Benzhydryl Compounds
  • Endocrine Disruptors
  • Environmental Pollutants
  • PPAR gamma
  • Phenols
  • bisphenol A