(E)4-[4-N,N-dimethylaminophenyl]but-3-en-2-one mitigates radiation-induced chromosome damage in BALB/c mouse bone marrow

Mutat Res Genet Toxicol Environ Mutagen. 2020 Oct-Dec:858-860:503238. doi: 10.1016/j.mrgentox.2020.503238. Epub 2020 Aug 13.

Abstract

We examined the effects of administration of (E) 4-[4-N,N-dimethylaminophenyl]but-3-en-2-one (DMAP) on radiation-induced chromosome damage in mice. Mice were whole-body exposed to γ-rays, 0-4 Gy, and then immediately administered DMAP, 20 mg/kg. After 24 h, mice were sacrificed, femora were removed, marrow was extracted, and chromosome aberrations were scored in the bone marrow cells. With vehicle-only (saline or oil) treatment, radiation dose-dependent damage was seen in aberrant cells, chromosome breaks, chromatid breaks, centric rings, di-, tri-, and tetracentrics, acentric fragments, total aberrations, polyploidy, and pulverization. Post-administration of DMAP was protective as it reduced chromosome damage. DMAP treatment may be a useful protective agent following radiation accidents or radiotherapy.

Keywords: Aberrant cells; Chromosome break; Dicentric.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aniline Compounds / pharmacology*
  • Animals
  • Bone Marrow / drug effects*
  • Bone Marrow / radiation effects
  • Bone Marrow Cells / drug effects*
  • Bone Marrow Cells / radiation effects
  • Butanones / pharmacology*
  • Chromosome Aberrations / drug effects*
  • Chromosome Aberrations / radiation effects
  • Chromosome Breakage / drug effects
  • Chromosome Breakage / radiation effects
  • Dose-Response Relationship, Radiation
  • Free Radical Scavengers / pharmacology
  • Gamma Rays
  • Male
  • Mice, Inbred BALB C
  • Radiation-Protective Agents / pharmacology*

Substances

  • Aniline Compounds
  • Butanones
  • Free Radical Scavengers
  • Radiation-Protective Agents
  • 4-(4-N,N-dimethylaminophenyl)but-3-en-2-one