Structure and gene cluster of the O-antigen of Escherichia coli strain SDLZB008

Andrei V Perepelov; Yajun Song; Yiming Zhu; Alexander S Shashkov; Andrei V Filatov; Bin Hu

doi:10.1016/j.carres.2020.108154

Structure and gene cluster of the O-antigen of Escherichia coli strain SDLZB008

Carbohydr Res. 2020 Dec:498:108154. doi: 10.1016/j.carres.2020.108154. Epub 2020 Sep 23.

Authors

Andrei V Perepelov¹, Yajun Song², Yiming Zhu², Alexander S Shashkov³, Andrei V Filatov³, Bin Hu⁴

Affiliations

¹ N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991, Moscow, Russian Federation. Electronic address: andreivperepelov@gmail.com.
² TEDA Institute of Biological Sciences and Biotechnology, Nankai University, TEDA, 300457, Tianjin, PR China.
³ N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991, Moscow, Russian Federation.
⁴ Shandong Center for Disease Control and Prevention, Jinan, 250014, Shandong, PR China.

PMID: 33197700
DOI: 10.1016/j.carres.2020.108154

Abstract

The O-polysaccharide (O-antigen) of Escherichia coli SDLZB008 was isolated from the lipopolysaccharide and studied by sugar analyses along with ¹H and ¹³C NMR spectroscopy. The following structure of the branched pentasaccharide repeating unit was established, which is unique among the known structures of bacterial polysaccharides: The O-antigen gene cluster of E. coli SDLZB008 has been sequenced. The gene functions were tentatively assigned by comparison with sequences in the available databases and found to be in full agreement with the O-polysaccharide structure.

Keywords: Bacterial polysaccharide structure; Escherichia coli; Lipopolysaccharide; O-antigen; O-antigen gene cluster.

MeSH terms

Carbohydrate Sequence
Escherichia coli / chemistry*
Escherichia coli / genetics*
Multigene Family / genetics*
O Antigens / chemistry*
O Antigens / genetics*

Substances

O Antigens