Unlike the entanglement of quantum states, very little is known about the entanglement of bipartite channels, called dynamical entanglement. Here we work with the partial transpose of a superchannel, and use it to define computable measures of dynamical entanglement, such as the negativity. We show that a version of it, the max-logarithmic negativity, represents the exact asymptotic dynamical entanglement cost. We discover a family of dynamical entanglement measures that provide necessary and sufficient conditions for bipartite channel simulation under local operations and classical communication and under operations with positive partial transpose.