ZIF-C for targeted RNA interference and CRISPR/Cas9 based gene editing in prostate cancer

Chem Commun (Camb). 2020 Dec 21;56(98):15406-15409. doi: 10.1039/d0cc06241c. Epub 2020 Nov 16.

Abstract

Gene therapy is highly suited for prostate cancer (PC). Metal-organic-frameworks (MOFs) are potential gene delivery systems. Target-specific cytoplasmic and nuclear knockdown in host gene expression using ZIF-C is shown for the first time through RNAi and CRISPR/Cas9 based gene editing in PC cells. A green tea phytochemical coating enhances intracellular delivery.

MeSH terms

  • CRISPR-Associated Protein 9 / antagonists & inhibitors*
  • CRISPR-Associated Protein 9 / genetics
  • Gene Editing
  • Gene Transfer Techniques
  • Humans
  • Male
  • Metal-Organic Frameworks / chemistry
  • Metal-Organic Frameworks / pharmacology*
  • PC-3 Cells
  • Prostatic Neoplasms / drug therapy*
  • Prostatic Neoplasms / genetics
  • RNA Interference / drug effects*

Substances

  • Metal-Organic Frameworks
  • CRISPR-Associated Protein 9