Computational Design of Multiplex Oligonucleotide-Based Assays

Michaela Hendling; Ivan Barišić

doi:10.1007/978-1-0716-0822-7_2

Computational Design of Multiplex Oligonucleotide-Based Assays

Methods Mol Biol. 2021:2189:19-30. doi: 10.1007/978-1-0716-0822-7_2.

Authors

Michaela Hendling¹, Ivan Barišić²

Affiliations

¹ Center for Health & Bioresources, Molecular Diagnostics, Austrian Institute of Technology GmbH, Vienna, Austria. Michaela.hendling@ait.ac.at.
² Center for Health & Bioresources, Molecular Diagnostics, Austrian Institute of Technology GmbH, Vienna, Austria.

PMID: 33180290
DOI: 10.1007/978-1-0716-0822-7_2

Abstract

The success of any oligonucleotide-based experiment strongly depends on the accurate design of the components. Oli2go is a user-friendly web tool that provides efficient multiplex oligonucleotide design including specificity and primer dimer checks. Its fully automated workflow involves important design steps that use specific parameters to produce high-quality oligonucleotides. This chapter describes how these steps are computationally implemented by oli2go.

Keywords: Multiplex oligonucleotide design; Primer dimers; Probe specificity.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Algorithms*
Oligonucleotides / genetics*
Sequence Analysis, DNA*
Software*

Substances

Oligonucleotides