Engineered trimeric ACE2 binds viral spike protein and locks it in "Three-up" conformation to potently inhibit SARS-CoV-2 infection

Liang Guo; Wenwen Bi; Xinling Wang; Wei Xu; Renhong Yan; Yuanyuan Zhang; Kai Zhao; Yaning Li; Mingfeng Zhang; Xia Cai; Shibo Jiang; Youhua Xie; Qiang Zhou; Lu Lu; Bobo Dang

doi:10.1038/s41422-020-00438-w

Engineered trimeric ACE2 binds viral spike protein and locks it in "Three-up" conformation to potently inhibit SARS-CoV-2 infection

Cell Res. 2021 Jan;31(1):98-100. doi: 10.1038/s41422-020-00438-w. Epub 2020 Nov 11.

Authors

Liang Guo^#^{1

2

3}, Wenwen Bi^#^{1

2

3}, Xinling Wang^#⁴, Wei Xu^#⁴, Renhong Yan^#^{1

2

3}, Yuanyuan Zhang^#^{1

2

3}, Kai Zhao^{1

2

3}, Yaning Li^{1

2

3}, Mingfeng Zhang^{1

2

3}, Xia Cai⁴, Shibo Jiang⁴, Youhua Xie⁴, Qiang Zhou^{5

6

7}, Lu Lu⁸, Bobo Dang^{9

10

11}

Affiliations

¹ Key Laboratory of Structural Biology of Zhejiang Province, School of Life Sciences, Westlake University, Hangzhou, Zhejiang, 310024, China.
² Center for Infectious Disease Research, Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou, Zhejiang, 310024, China.
³ Institute of Biology, Westlake Institute for Advanced Study, Hangzhou, Zhejiang 310024, China.
⁴ Key Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), School of Basic Medical Sciences and Biosafety Level 3 Laboratory, Fudan University, Shanghai, 200032, China.
⁵ Key Laboratory of Structural Biology of Zhejiang Province, School of Life Sciences, Westlake University, Hangzhou, Zhejiang, 310024, China. zhouqiang@westlake.edu.cn.
⁶ Center for Infectious Disease Research, Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou, Zhejiang, 310024, China. zhouqiang@westlake.edu.cn.
⁷ Institute of Biology, Westlake Institute for Advanced Study, Hangzhou, Zhejiang 310024, China. zhouqiang@westlake.edu.cn.
⁸ Key Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), School of Basic Medical Sciences and Biosafety Level 3 Laboratory, Fudan University, Shanghai, 200032, China. lul@fudan.edu.cn.
⁹ Key Laboratory of Structural Biology of Zhejiang Province, School of Life Sciences, Westlake University, Hangzhou, Zhejiang, 310024, China. dangbobo@westlake.edu.cn.
¹⁰ Center for Infectious Disease Research, Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou, Zhejiang, 310024, China. dangbobo@westlake.edu.cn.
¹¹ Institute of Biology, Westlake Institute for Advanced Study, Hangzhou, Zhejiang 310024, China. dangbobo@westlake.edu.cn.

^# Contributed equally.

No abstract available

Publication types

Letter
Research Support, Non-U.S. Gov't

MeSH terms

Angiotensin-Converting Enzyme 2 / chemistry*
Angiotensin-Converting Enzyme 2 / pharmacology
COVID-19 / metabolism
COVID-19 Drug Treatment*
Humans
Mutation, Missense*
Protein Binding
Protein Engineering
Protein Multimerization*
SARS-CoV-2 / chemistry*
SARS-CoV-2 / metabolism

Substances

ACE2 protein, human
Angiotensin-Converting Enzyme 2