P-glycoprotein (ABCB1) is involved in vitamin K efflux

Food Chem. 2021 May 1:343:128510. doi: 10.1016/j.foodchem.2020.128510. Epub 2020 Nov 1.

Abstract

ABCB1 (P-glycoprotein/MDR1) is a multidrug efflux transporter that has previously been involved in cholesterol and vitamin D metabolism. Our aim was to explore whether ABCB1 is also involved in vitamin K efflux. Vitamin K apical efflux was significantly decreased in presence of ABCB1 inhibitor in Caco-2 cells (-20.4%; p < 0.05) and increased in Griptite cells overexpressing ABCB1 (+40.7%; p < 0.05). In vivo, the vitamin K postprandial response was higher in male Abcb1-/- mice after gavage compared to control animals (+115%; p < 0.05), but was unchanged in female mice. Finally, a vitamin K transintestinal efflux and a biliary vitamin K efflux were observed, but the specific involvement of ABCB1 could not be confirmed in these pathways. Overall, we showed for the first time that ABCB1 is involved in enterocyte vitamin K efflux in both cell and mouse models and regulates vitamin K absorption in mice.

Keywords: Bile; Bioavailability; Enterocyte; Intestine; MDR1; Phylloquinone.

MeSH terms

  • ATP Binding Cassette Transporter, Subfamily B, Member 1 / genetics
  • ATP Binding Cassette Transporter, Subfamily B, Member 1 / metabolism*
  • Animals
  • Caco-2 Cells
  • Enterocytes / drug effects
  • Enterocytes / metabolism
  • Female
  • Humans
  • Male
  • Mice
  • Mice, Mutant Strains
  • Postprandial Period
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism
  • Vitamin K / pharmacokinetics*

Substances

  • ATP Binding Cassette Transporter, Subfamily B, Member 1
  • Recombinant Proteins
  • Vitamin K