Characterization of glycosphingolipids from gastrointestinal stromal tumours

Licínia Santos; Chunsheng Jin; Taťána Gazárková; Anders Thornell; Olov Norlén; Karin Säljö; Susann Teneberg

doi:10.1038/s41598-020-76104-3

Characterization of glycosphingolipids from gastrointestinal stromal tumours

Sci Rep. 2020 Nov 9;10(1):19371. doi: 10.1038/s41598-020-76104-3.

Authors

Licínia Santos¹, Chunsheng Jin¹, Taťána Gazárková¹, Anders Thornell^{2

3}, Olov Norlén⁴, Karin Säljö^{3

5}, Susann Teneberg⁶

Affiliations

¹ Department of Medical Biochemistry and Cell Biology, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, P.O. Box 440, 405 30, Gothenburg, Sweden.
² Department of Surgery, Institute of Clinical Sciences, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
³ Region Västra Götaland, Sahlgrenska University Hospital, Gothenburg, Sweden.
⁴ Department of Surgical Sciences, Uppsala University, Uppsala University Hospital, Uppsala, Sweden.
⁵ Department of Plastic Surgery, Institute of Clinical Sciences, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
⁶ Department of Medical Biochemistry and Cell Biology, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, P.O. Box 440, 405 30, Gothenburg, Sweden. Susann.Teneberg@medkem.gu.se.

Abstract

Gastrointestinal stromal tumours (GISTs) are the major nonepithelial neoplasms of the human gastrointestinal tract with a worldwide incidence between 11 and 15 per million cases annually. In this study the acid and non-acid glycosphingolipids of three GISTs were characterized using a combination of thin-layer chromatography, chemical staining, binding of carbohydrate recognizing ligands, and mass spectrometry. In the non-acid glycosphingolipid fractions of the tumors globotetraosylceramide, neolactotetraosylceramide, and glycosphingolipids with terminal blood group A, B, H, Le^x, Le^a, Le^y and Le^b determinants were found. The relative amounts of these non-acid compounds were different in the three tumour samples. The acid glycosphingolipid fractions had sulfatide, and the gangliosides GM3, GD3, GM1, Neu5Acα3neolactotetraosylceramide, GD1a, GT1b and GQ1b. In summary, we have characterized the glycosphingolipids of GISTs and found that the pattern differs in tumours from different individuals. This detailed characterization of glycosphingolipid composition of GISTs could contribute to recognition of new molecular targets for GIST treatment and sub-classification.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Gastrointestinal Neoplasms
Gastrointestinal Stromal Tumors / metabolism*
Glycosphingolipids / metabolism*
Humans

Substances

Glycosphingolipids