Magnetic solid-phase extraction of gingerols in ginger containing products

Lidia Akemi Akamine; Deyber Arley Vargas Medina; Fernando Mauro Lanças

doi:10.1016/j.talanta.2020.121683

Magnetic solid-phase extraction of gingerols in ginger containing products

Talanta. 2021 Jan 15:222:121683. doi: 10.1016/j.talanta.2020.121683. Epub 2020 Sep 17.

Authors

Lidia Akemi Akamine¹, Deyber Arley Vargas Medina¹, Fernando Mauro Lanças²

Affiliations

¹ University of São Paulo, Institute of Chemistry of São Carlos, SP, Brazil.
² University of São Paulo, Institute of Chemistry of São Carlos, SP, Brazil. Electronic address: flancas@iqsc.usp.br.

PMID: 33167289
DOI: 10.1016/j.talanta.2020.121683

Abstract

In this study, a graphene oxide/magnetite (GO-Fe₃O₄) nanocomposite was synthesized and used as a sorbent in the magnetic solid-phase extraction (MSPE) of gingerols from fresh ginger rhizomes, ginger extracts, commercial tea samples, ginger candies, thermogenic supplements, and tonic water. An MSPE method was developed, and the main influencing parameters in the sample preparation process were investigated. After GO-Fe₃O₄ based MSPE, 6-gingerol, 8-gingerol, and 10-gingerol were determined by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The whole GO-Fe₃O₄-MSPE-LC-MS/MS method proved high selectivity and consistent analytical confidence. The limits of detection (LOD) ranged between 2 and 3 μg L-1. Intra-day and inter-day RSDs fluctuated between 1.7 - 13.4% and 0.4-10.9%, respectively. Weighted calibration revealed good linearity within the studied range (5-200 μg L^-1) and guaranteed appropriate accuracy (relative residues < 25%). MSPE with GO-Fe₃O₄ demonstrated to be a practical, fast, efficient, high-throughput, and environmental-friendly sample preparation technique for determining of gingerols in commercial products, and its hyphenation with LC-MS/MS analysis yield a valuable analytical tool for the confident quality control of commercial ginger-containing products.

Keywords: Ginger; Gingerols; Graphene oxide; Liquid chromatography; Magnetic solid-phase extraction; Magnetite; Mass spectrometry.