Analysis and Isolation of Mouse Leukemic Stem Cells

Fang Dong; Haitao Bai; Hideo Ema

doi:10.1007/978-1-0716-0810-4_4

Analysis and Isolation of Mouse Leukemic Stem Cells

Methods Mol Biol. 2021:2185:51-63. doi: 10.1007/978-1-0716-0810-4_4.

Authors

Fang Dong¹, Haitao Bai¹, Hideo Ema²

Affiliations

¹ State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, China.
² Department of Regenerative Medicine, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, China. hema@ihcams.ac.cn.

PMID: 33165842
DOI: 10.1007/978-1-0716-0810-4_4

Abstract

Flow cytometry has been widely used in basic and clinical research for analysis of a variety of normal and malignant cells. Hematopoietic stem cells (HSCs) and leukemic stem cells (LSCs) can be highly purified by flow cytometry. Isolated HSCs and LSCs can be functionally identified by transplantation assays and can also be studied at the molecular level. Here we describe the flow cytometry methods for analysis and isolation of mouse HSCs and LSCs.

Keywords: Flow cytometry; Hematopoietic stem cells (HSCs); Immunophenotype; Leukemic stem cells (LSCs ).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Flow Cytometry*
Hematopoietic Stem Cells* / metabolism
Hematopoietic Stem Cells* / pathology
Leukemia* / metabolism
Leukemia* / pathology
Mice
Neoplastic Stem Cells* / metabolism
Neoplastic Stem Cells* / pathology