Measurement of Cellulase and Xylanase Activities in Trichoderma reesei

Methods Mol Biol. 2021:2234:135-146. doi: 10.1007/978-1-0716-1048-0_12.

Abstract

The microbial cellulase system is responsible for the generation of glucose from cellulose. Cellulases are comprised of at least three major groups of enzymes, namely endoglucanases, exoglucanases, and β-glucosidases. On the other hand, xylanases function in the degradation of hemicellulose and work synergistically with cellulases for the degradation of lignocellulosic biomass. Here, we describe the most commonly used methods for the activity measurement of cellulases and xylanases.

Keywords: Cellulase; Lignocellulosic biomass; Measurement of enzyme activity; Xylanase.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biological Assay / methods*
  • Cellulase / metabolism*
  • Endo-1,4-beta Xylanases / metabolism*
  • Glucose / metabolism
  • Hypocreales / enzymology*
  • Reference Standards
  • Xylose / metabolism
  • beta-Glucosidase / metabolism

Substances

  • Xylose
  • beta-Glucosidase
  • Cellulase
  • Endo-1,4-beta Xylanases
  • Glucose

Supplementary concepts

  • Trichoderma reesei