This chapter explains how to perform a batch cultivation of Trichoderma reesei in bench top bioreactors, exemplarily using wheat straw as sole carbon source, and a selection of recommended, frequently used analyses to monitor the cultivation (intra- and extracellular as well), which are microscopic analysis, sodium hydroxide soluble protein, Bradford assay, and GC analysis.
Keywords: Batch cultivation; Bioreactor; Bradford assay; Erythritol; Microscopic analysis; Sodium hydroxide soluble protein; Trichoderma reesei.